[42] Immunohistochemistry (IHC) Flashcards
What is the fundamental principle of Immunohistochemistry (IHC)?
It involves using specific antibodies to detect particular antigens in tissue sections.
Initials: IHC
Immunohistochemistry
What are the key steps involved in IHC?
- Tissue preparation
- Antigen retrieval
- Blocking
- Primary antibody incubation
- Secondary antibody incubation
- Detection
How does IHC differ from Immunocytochemistry (ICC)?
IHC is performed on tissue sections whereas ICC is performed on cells.
What are the labels used in IHC?
- Enzymes
- Fluorescent dyes
What is the role of a secondary antibody in IHC?
It binds to the primary antibody and carries the detectable label.
Why is blocking used in IHC?
It prevents non-specific binding of antibodies.
What is the importance of antigen retrieval in IHC?
It unmask hidden antigen sites in the tissue sections.
Why are tissue sections used in IHC?
To observe the distribution and localization of biomolecules in the biological structures.
What are some applications of IHC?
- Disease diagnosis
- Drug testing
- Research in cellular biology
What are some limitations of IHC?
- Non-specific staining
- Variability due to different protocols
- Difficulty in quantifying the results
