[37] Immunoassays Flashcards
What is the fundamental principle of an immunoassay?
Detection and quantification of antigens or antibodies through their specific immunological reaction.
What does an immunoassay involve?
- Binding of antigen and antibody
- Signal detection
- Signal amplification
Initials: ELISA
Enzyme-Linked Immunosorbent Assay
What is the purpose of an ELISA in immunoassays?
To detect and measure antibodies in a sample.
Initials: RIA
Radioimmunoassay
What is the purpose of a RIA in immunoassays?
To measure concentrations of antigens by use of antibodies and radioisotopes.
Initials: FIA
Fluoroimmunoassay
What is the purpose of a FIA in immunoassays?
To detect and quantify substances such as peptides, proteins, antibodies, and hormones in a sample.
What are the key components of an immunoassay?
- The antigen or antibody
- The detection method
- The signal amplification strategy
In what fields are immunoassays commonly used?
- Clinical diagnostics
- Pharmacology
- Environmental testing
What are the main types of immunoassays?
- ELISA
- RIA
- FIA
What makes immunoassays a preferred method in various fields?
- High specificity
- High sensitivity
- Broad applicability
What are some limitations of immunoassays?
- Cross-reactivity
- False positive/negative results
- Time-consuming procedure
What is the purpose of a FIA in immunoassays?
To detect and quantify substances such as peptides, proteins, antibodies, and hormones in a sample.
