[29] DNA Microarray Flashcards
What is a DNA Microarray?
A technology used to detect the expression levels of thousands of genes simultaneously.
What is the main application of DNA Microarray?
Gene expression profiling.
What is gene expression profiling?
The measurement of the activity (expression) of thousands of genes at once.
How does a DNA Microarray work?
By measuring the amount of hybridization between DNA samples and probes attached to the microarray.
What are the key components of a DNA Microarray?
- Microscopic DNA spots
- Solid surface (glass, plastic)
- DNA probes
What is a DNA spot in a DNA Microarray?
A location on the microarray surface where a specific DNA sequence is attached.
What is a DNA probe?
A fragment of DNA used to detect the presence of a specific DNA sequence in a sample.
How is the data from a DNA Microarray analyzed?
By using bioinformatics software to interpret the pattern of gene expression.
What does a DNA Microarray reveal about a gene?
The level of expression or activity of that gene in different conditions or tissues.
What fields commonly use DNA Microarrays?
- Genomics
- Oncology
- Pharmacogenomics
- Developmental biology
How do DNA Microarrays aid in genomics?
They allow for the analysis of gene expression across the entire genome.
What is the role of DNA Microarrays in oncology?
They are used to understand the gene expression patterns in different types of cancer.
How do DNA Microarrays contribute to pharmacogenomics?
They help identify how different genes affect an individual’s response to drugs.
How does developmental biology benefit from DNA Microarrays?
They enable the study of gene expression changes during the development of organisms.
What is hybridization in the context of DNA Microarrays?
The binding of a DNA sample to its complementary DNA probe on the microarray.
