[32] Cell Culture Techniques Flashcards
What is Cell Culture?
A process where cells are grown under controlled conditions, usually outside their natural environment.
List some types of cell culture techniques.
- Monolayer culture
- Suspension culture
What is Monolayer Culture?
A method where cells are grown in a single layer on a flat surface.
What is Suspension Culture?
A method where cells are grown in a liquid medium and are free-floating.
What are the essential components of a cell culture medium?
- Amino acids
- Vitamins
- Salts
- Glucose
What is the role of a cell culture medium?
To provide the necessary nutrients and environment for cell growth and survival.
What is Cell Line?
A genetically uniform type of cell that can proliferate indefinitely in culture.
What is Primary Cell Culture?
A culture started from cells, tissues or organs taken directly from organisms.
What is an Inoculum in cell culture?
It’s the initial set of cells used to start a cell culture.
What are the basic steps in a cell culture process?
- Inoculation
- Incubation
- Maintenance
- Examination
- Preservation
What is the purpose of incubation in cell culture?
To provide the cells with the optimal conditions for growth.
What is the goal of maintenance in cell culture?
To keep the cells healthy and growing by providing fresh medium and optimal conditions.
Why is examination important in cell culture?
To check for contamination and monitor cell health and growth.
What is the purpose of preservation in cell culture?
To store and protect cells for future use.
What does Aseptic Technique mean in cell culture?
It is a set of practices to prevent contamination of the cell culture.
