[33] Mammalian Cell Culture Flashcards
What is Mammalian Cell Culture?
A process where cells from mammals are grown and maintained in a controlled environment.
List the types of mammalian cell cultures.
- Primary cell culture
- Secondary (cell line) culture
- Cell strain culture
What is Primary Cell Culture in mammalian cell culture?
Culture derived directly from cells of a mammalian tissue.
What is Secondary Cell Culture in mammalian cell culture?
A culture derived from the primary culture that has been sub-cultured.
What is Cell Strain Culture in mammalian cell culture?
Cells derived from a primary culture that have been sub-cultured and developed specific mutations.
What are the requirements for mammalian cell culture?
- Sterile environment
- Appropriate temperature and gas mixture
- Nutrient-rich medium
Why is a sterile environment necessary in mammalian cell culture?
To prevent contamination that can affect cell growth and function.
What temperature is commonly used for mammalian cell culture?
37 degrees Celsius (body temperature of most mammals).
What is the role of the nutrient-rich medium in mammalian cell culture?
To supply the necessary nutrients for cell growth and survival.
What is a Serum in the context of mammalian cell culture?
It is a supplement used in the growth medium that provides additional nutrients and growth factors.
What is the purpose of a CO2 incubator in mammalian cell culture?
It maintains the optimal temperature and CO2 concentration for cell growth.
What are the stages involved in mammalian cell culture?
- Cell seeding
- Cell attachment
- Cell growth
- Sub-culturing
What does Cell Seeding mean in mammalian cell culture?
The process of placing the cells in a culture vessel along with culture medium.
What does Cell Attachment mean in mammalian cell culture?
The process where cells adhere to the surface of the culture vessel.
What does Sub-culturing mean in mammalian cell culture?
The process of transferring the cells from an old vessel to a new one to maintain growth.
What are Anchorage-dependent cells in mammalian cell culture?
Cells that require a surface to attach to for growth.
What are Anchorage-independent cells in mammalian cell culture?
Cells that do not require a surface to attach to for growth and can grow in suspension.
What is the Trypsinization process in mammalian cell culture?
The use of the enzyme trypsin to detach cells from the surface of the culture vessel during sub-culturing.
What is the importance of pH in mammalian cell culture?
pH influences cell metabolism and function, and needs to be controlled for optimal cell growth.
What are the common types of mammalian cells used in cell culture?
- Fibroblasts
- Epithelial cells
- Lymphocytes
