[38] Immunocytochemistry (ICC) Flashcards
What is the fundamental principle of Immunocytochemistry (ICC)?
Identification of cellular or subcellular components (proteins, antigens) by using specific antibodies tagged with a visible label.
Initials: ICC
Immunocytochemistry
What are the main steps involved in ICC?
- Fixation
- Permeabilization
- Blocking
- Primary antibody incubation
- Secondary antibody incubation
- Detection
How does ICC differ from Immunohistochemistry (IHC)?
ICC is performed on cells whereas IHC is performed on tissue sections.
What are the labels used in ICC?
- Fluorescent dyes
- Enzymes
What is the purpose of using a secondary antibody in ICC?
It binds to the primary antibody and carries the detectable label.
What is the purpose of blocking in ICC?
To prevent non-specific binding of antibodies.
Why is fixation important in ICC?
It preserves cell morphology and immobilizes the target antigens.
Why is permeabilization performed in ICC?
It increases accessibility of antibodies to intracellular antigens.
What are some applications of ICC?
- Cellular localization of proteins
- Analysis of cell morphology
- Diagnosis of diseases
What are some limitations of ICC?
- Non-specific staining
- High background noise
- Difficulty in antibody penetration
