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[21] Introduction to Molecular Biology > [39] Immunostaining > Flashcards

[39] Immunostaining Flashcards

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1
Q

What does immunostaining refer to?

A

A method in microscopy for detecting specific proteins in cells or tissues.

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2
Q

List the dyes used in immunostaining by their color.

A
  • Purple
  • Blue
  • Green
  • Orange
  • Red
  • DAPI
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3
Q

What is the nm range for the purple dye in immunostaining?

A

432 nm

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4
Q

What is the nm range for the blue dye in immunostaining?

A

480 nm

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5
Q

What is the nm range for the green dye in immunostaining?

A

488 nm

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6
Q

What is the nm range for the orange dye in immunostaining?

A

568 nm

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7
Q

What is the nm range for the red dye in immunostaining?

A

647 nm

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8
Q

What is the nm range for DAPI in immunostaining?

A

DAPI typically absorbs light at approximately 358 nm and emits light at approximately 461 nm

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9
Q

What is FITC used for in immunostaining?

A

Fluorescein isothiocyanate (FITC) is used to stain and visualize proteins.

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10
Q

What is TRITC used for in immunostaining?

A

Tetramethylrhodamine (TRITC) is used as a red fluorescent dye conjugated to antibodies for use in immunofluorescence microscopy.

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11
Q

What are Cyanines used for in immunostaining?

A

Cyanines are synthetic dyes used to stain nucleic acids.

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12
Q

What is the function of DAPI in immunostaining?

A

DAPI is used to stain DNA in fluorescence microscopy.

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13
Q

List the dyes used in immunostaining by their name.

A
  • FITC
  • TRITC/Rhodamine
  • Cyanines
  • DAPI
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14
Q

What is the color of the dye Fluorescein Isothiocyanate (FITC) in immunostaining?

A

Green

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15
Q

What is the color of the dye Tetramethylrhodamine (TRITC) in immunostaining?

A

Red

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16
Q

What is the color of the dye Cyanines in immunostaining?

A

Varies depending on the specific cyanine dye used; can range from blue to near-infrared

17
Q

What is the color of the dye DAPI in immunostaining?

A

Blue

18
Q

What is Rhodamine in the context of immunostaining?

A

Rhodamine is a family of dyes used in biology for fluorescent microscopy. Tetramethylrhodamine (TRITC) is a red-fluorescing derivative of rhodamine.

[21] Introduction to Molecular Biology (53 decks)

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