[34] Bacterial Cell Culture Flashcards
What is Bacterial Cell Culture?
It’s the process of growing bacteria under controlled conditions.
List the types of media used in bacterial cell culture.
- Nutrient Broth
- Agar plates
- Enriched media
- Selective media
- Differential media
What is Nutrient Broth used for in bacterial cell culture?
For growing a wide variety of non-fastidious organisms.
What is the role of Agar Plates in bacterial cell culture?
Used to isolate and identify specific types of bacteria.
What is the purpose of Enriched media in bacterial cell culture?
To grow nutritionally demanding bacteria.
What is Selective media used for in bacterial cell culture?
To inhibit the growth of certain types of bacteria while promoting the growth of others.
What is Differential media in bacterial cell culture?
Used to differentiate between closely related bacteria or groups of bacteria.
Why is a sterile environment crucial in bacterial cell culture?
To prevent contamination from foreign microorganisms.
What is a Bacterial Colony in bacterial cell culture?
A visible group of bacteria originating from a single mother cell.
How does temperature influence bacterial cell culture?
Each bacterial species has an optimal temperature range for growth.
What is Incubation in bacterial cell culture?
The process of maintaining a controlled environment to promote bacterial growth.
What is the purpose of Streaking in bacterial cell culture?
To isolate a pure bacterial culture from a mixed culture.
How does pH affect bacterial cell culture?
It influences bacterial metabolism and growth, with each species having an optimal pH range.
What are Autotrophic bacteria?
Bacteria that can synthesize their own food from inorganic substances.
What are Heterotrophic bacteria?
Bacteria that obtain nutrients from organic substances.
