[41] Fluorescence In Situ Hybridization (FISH)

Question 1

What does the acronym FISH stand for?

Answer 1

Fluorescence In Situ Hybridization

Question 2

What is the primary purpose of the FISH technique?

Answer 2

Detection and localization of specific DNA or RNA sequences

Question 3

Is FISH utilized in the field of cytogenetics or proteomics?

Answer 3

Cytogenetics

Question 4

Can FISH be applied to both metaphase chromosomal spreads and interphase nuclei?

Answer 4

Yes

Question 5

In the context of FISH, what does ‘in situ’ mean?

Answer 5

The technique is applied directly on chromosomes inside the cell

Question 6

Is FISH employed for research, clinical practice, or both?

Answer 6

Both research and clinical practice

Question 7

Does FISH allow detection of gene mutations, deletions, duplications, or all of these?

Answer 7

Gene mutations, deletions, and duplications

Question 8

Can FISH be used to identify chromosomal abnormalities in cancer cells?

Answer 8

Yes

Question 9

Does the FISH technique involve the use of fluorescently labeled probes?

Answer 9

Yes

Question 10

What is the role of the probe in FISH?

Answer 10

It binds to the specific DNA sequence under study

Question 11

How is the probe visualized in FISH?

Answer 11

By using a fluorescence microscope

Question 12

Can FISH be used to detect single nucleotide variations?

Answer 12

No, it’s not typically used for such small variations