[03] RNA Transcription and Translation Flashcards

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1
Q

What is the first step in gene expression?

A

Transcription

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2
Q

Where does transcription occur in a eukaryotic cell?

A

Nucleus

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3
Q

What is the product of transcription?

A

RNA

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4
Q

What enzyme synthesizes RNA during transcription?

A

RNA polymerase

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5
Q

What are the three stages of transcription?

A
  • Initiation
  • Elongation
  • Termination
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6
Q

What is the role of promoter in transcription?

A

A DNA sequence that signals the start of the gene and the site for RNA polymerase to bind

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7
Q

How does RNA polymerase know where to stop transcription?

A

It recognizes specific DNA sequences called termination signals

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8
Q

What is RNA splicing?

A

The process of removing introns and joining exons in a pre-mRNA molecule

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9
Q

What are introns and exons?

A
  • Introns → non-coding sequences
  • Exons → coding sequences
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10
Q

What happens during translation?

A

The mRNA is decoded to produce a specific protein

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11
Q

Where does translation occur in a eukaryotic cell?

A

Cytoplasm

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12
Q

What are the three types of RNA involved in translation?

A
  • mRNA → messenger RNA
  • rRNA → ribosomal RNA
  • tRNA → transfer RNA
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13
Q

What is the role of tRNA in translation?

A

It brings amino acids to the ribosome based on the codon sequence in mRNA

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14
Q

What is a codon?

A

A sequence of three nucleotides in mRNA that specifies a particular amino acid

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15
Q

What is an anticodon?

A

A sequence of three nucleotides in tRNA that pairs with a specific codon in mRNA

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16
Q

What is the start codon and what does it code for?

A

AUG, it codes for Methionine

17
Q

What are the three stop codons?

A
  • UAA
  • UAG
  • UGA
18
Q

What are the three stages of translation?

A
  • Initiation
  • Elongation
  • Termination
19
Q

What is a polysome?

A

A complex of an mRNA molecule and two or more ribosomes that act to form polypeptide chains during active translation

20
Q

What is the role of a ribosome during translation?

A

It facilitates the coupling of tRNA anticodons with mRNA codons during protein synthesis