[44] Methods Flashcards
1
Q
What mouse model did the researchers generate?
A
One with conditional presentation of an antigen (ovalbumin) on activated cardiac fibroblasts.
2
Q
What cells were introduced through adoptive transfer?
A
CD8+ T cells with a cognate T-cell receptor against ovalbumin.
3
Q
How was FAP identified as a potential target antigen?
A
From an RNAseq database of human heart transplant donor and recipient left ventricular tissue.
4
Q
What model was used for hypertensive cardiac injury and fibrosis in mice?
A
Angiotensin II and phenylephrine (AngII/PE) model.
