[30] Single-cell Sequencing Platform Utility Flashcards
1
Q
What does Single-Cell Sequencing involve?
A
Isolating and barcoding cells before sequencing.
2
Q
What is Droplet Sequencing?
A
Partitioning single cells or nuclei in microfluidic devices with beads, cell barcodes, and UMIs.
3
Q
What is UMI?
A
Unique Molecular Identifier
4
Q
How does Smart-Seq3 differ from Droplet Sequencing?
A
- Fewer cells per experiment
- Full-length transcripts
- Detects more genes per cell
5
Q
What is SPLiT-seq?
A
Repeatedly splitting and pooling samples for sequential molecular barcoding.
6
Q
What does DroNc-seq focus on?
A
Isolating nuclei from tissue.
7
Q
What is Spatial Transcriptomics?
A
Techniques like Slide-seq2 and Visium that allow for tissue dissociation on slides with spatially coded beads.
