[38] General Concepts and Methodology

Question 1

What is Transcriptional and Cellular Diversity in the human heart?

Answer 1

Reliance on specialized cell types and gene expression for function.

Question 2

Define Single Nuclear RNA Sequencing.

Answer 2

High-resolution technology for decoding individual transcriptional profiles of heart cells.

Question 3

snRNA-seq - Initials

Answer 3

Single Nuclear RNA Sequing

Question 4

What methodology was used for single nuclear RNA sequencing?

Answer 4

Microfluidic encapsulation, barcoding

Question 5

How were the transcriptional profiles analyzed?

Answer 5

Through highly variable genes; clustering of individual nuclear transcriptomes.

Question 6

What did the researchers examine in the transcriptional profiles?

Answer 6

Between-chamber and between-sex differential gene expression, genetic and pharmacologic data intersection.

Question 7

What is the focus of traditional cardiac research?

Answer 7

Cardiomyocytes for contractile functionality

Question 8

What are Cardiomyocytes?

Answer 8

Cells responsible for heart’s contractile functionality

Question 9

What is Transcriptional Analysis?

Answer 9

Analysis of RNA species’ transcription to define cell types and states.

Question 10

What recent advances have allowed more specific cell type identification?

Answer 10

Microfluidic encapsulation and cellular barcoding in single-cell RNA sequencing.