[37] Future and Conclusion

Question 1

What limitations do single-cell analysis tools have?

Answer 1

• Low per-cell transcript complexity
• Technical pitfalls
• Issues in documenting biological relevance of cellular subpopulations and signatures.

Question 2

What advancements could we see in the future of this field?

Answer 2

• Assessments of chromatin accessibility
• Chromatin conformation
• Histone modifications
• New modalities like sci-CAR and SHARE-seq

Question 3

sci-CAR - Initials

Answer 3

Single-cell Combinatorial Indexed Regulation

Question 4

SHARE-seq - Initials

Answer 4

Single-cell RNA-seq by Hashing

Question 5

What could spatial transcriptomics offer?

Answer 5

Insights into responses following injury and local alterations.