[31] Quality Control in Single-cell Sequencing

Question 1

What is crucial for scRNA-seq analysis?

Answer 1

Quality control by verifying ‘cell barcodes’.

Question 2

What variables can create interpretation challenges in scRNA-seq?

Answer 2

• Number of transcripts
• Number of genes
• Proportion of mitochondrial genes

Question 3

What could an increase in mitochondrial genes indicate?

Answer 3

Contamination or greater mitochondrial content/activation.

Question 4

What could high RNA abundance in cells indicate?

Answer 4

Larger cell size.

Question 5

What does ‘Ambient’ gene expression refer to?

Answer 5

Transcripts from other lysed cells contaminating the target cell.