XI Chap 8 Cell: Unit of Life Flashcards
_________ emphasized the unity underlying the diversity in forms of biological organisms
Cell theory
The physico-chemical approach to study of living organisms is called __________________
Reductionist Biology
_______ was an outstanding figure in the field of protein structure and founder of the ‘Madras’ school of conformational analysis of biopolumers
G N Ramachandran
____________ discovered the triple helical structure of collagen
G N Ramachandran
G N Ramachandran was deeply influenced by ______’s publications on models of the alpha-helix and Beta-sheet structures
Linus Pauling
______ is the basic unit of life in all organisms
Cell
___________ organisms are capable of independent existence and performing the essential functions of life
Unicellular organisms
_______ first saw and described a live cell
Anton Von Leeuwenhoek
_________ discovered the nucleus
Robert Brown
The invention of _________ and _________ revealed all the structural details of a cell
microscope and electron microscope
In ______ a German botanist named _______ observed that all plants are composed of different kids of cells
1838, Matthias Schleiden
In ________ a British Zoologist _________ studied animal cells and reported that cells had a thin outer layer today known as _________
1839,
Theodore Schwann,
plasma membrane
_________ concluded that the presence of cell wall is unique to plant cells
Theodore Schwann
Theodore Schwann proposed what hypothesis?
that the bodies of animals and plants are composed of cells and products of cells
_______ and _______ together formulated cell theory, What was lacking in this theory?
Schleiden and Schwann,
lacking - explanation for how new cells are formed
_____________ explained for the first time in the year ______ that cells divided and new cells are formed from pre-existing ones
Rudolf Virchow, 1855
What does omnis cellula-e cellula mean?
Every cell from the cell,
cells divide to form new cells
What are the 2 tenets of cell theory?
- All living organisms are composed of cells and products of cells
- All cells arise from pre-existing cells
What are the exceptions to cell theory?
Bacteria, Viruses, Sieve tube (enucleated), RBCs (enucleated), Rhizopus (multinucleated)
Cells of human cheek have ________ as delimiting structure of the cell, whereas onion cell has ______
outer membrane, cell wall
Cell is a ___________ that separates outer and inner environments so that _____________
chamber, reactions can continue unperturbed
EVERY cell at some point has DNA which stores info for some reactions and processes inside the cell. T or F?
True.
Even RBCs and sieve cells first have DNA then lose it to become enucleated
Cells that have membrane bound nuclei are called _____ whereas those that lack it are called _____
eukaryotic,
prokaryotic
Cell membrane is made up of ___________ which makes it __________ (hydrophilic/hydrophobic)
lipid, hydrophobic
Only in eukaryotic cells with membrane bound nuclei, a semi-fluid matrix called cytoplasm occupies the volume of the cell. T or F?
False, in both eukaryotic and prokaryotic
Prokaryote and eukaryotic cells BOTH have organelles. T or F?
True, but prokaryotes have non-membrane bound organelles
Main arena of cellular activities in both plant and animal cells is _________
cytoplasm
Various chemical reactions in the cytoplasm of a cell keep it in a _________ state
living
In addition to a nucleus, eukaryotic cells have other membrane-bound structures called ______
organelles
Endoplasmic reticulum, golgi complex, lysosomes, mitochondria, microbodies and vacuoles are all examples of ______________
cell organelles
Prokaryotic cells lack membrane bound organelles. T or F?
True
Ribosomes are membrane-bound organelles found in all cells - eukaryotic and prokaryotic. T or F?
False, found in all cells yes but NON-membrane bound
Ribosomes are found where in the cell?
Cytoplasm, within chloroplasts (in plants), within mitochondria and on rough ER
(in bacteria - associated with plasma membrane)
What is centrosome?
Non-membrane bound organelle,
in animal cells,
helps in cell division
Cells differ greatly in ______, ______ and ______
size, shape and activities
___________ are the smallest cells only _____ in length
Mycoplasma, 0.3 µm (micrometer)
Mycoplasma has ______ (cell wall / no cell wall) and its shape is ________
no cell wall, not fixed
Length of bacteria is ________
3 to 5 µm
Largest isolated single cell is _________
egg of an ostrich
Size of typical eukaryotic cell?
10-20 micrometers
Size of viruses?
0.02-0.2 micrometer
Size and full-form of PPLO?
0.1 micrometer
Pleuro Pneumonia Like Organisms
Human RBCs are about 7.0 µm in radius. T or F?
False, in diameter
_______ cells are some of the longest cells in multicellular organisms
Nerve
Match the following:
- round and biconcave
- branched and long
- elongated
- round and oval
- long and narrow
- amoeboid
Columnar epithelial cells, Tracheid, Mesophyll, RBC, WBC, Nerve cells
- round and biconcave - RBC
- branched and long - nerve cells
- elongated - tracheid
- round and oval - mesophyll
- long and narrow - columnar
- amoeboid - WBC
____ (____m) and _____ (_____m) units are visible in electron microscope but not in compound microscope
Nanometer 10^-9
Angstrom 10^-10
____ (____m) and _____ (_____m) units are visible in compound microscope
Micrometer 10^-6
Millimeter 10^-3
Some eukaryotic cells measure in millimeters. T or F?
True
Shapes of cells vary with the functions they perform. T or F?
True
Blue green algae and PPLO are prokaryotic cells. T or F?
True
Pick which of these are correct:
Prokaryotic cells are ________ compared to eukaryotic
smaller, have nuclear membrane, multiply rapidly, unique inclusions, no organelles at all
smaller, multiply rapidly, unique inclusions
4 basic shapes of bacteria?
Bacillus - rod
Coccus - spherical
Vibrio - comma
Spirillum - spiral
Shapes, functions and fundamental organisation of prokaryotic cells vary greatly. T or F?
False,
shapes and functions vary greatly
but fundamental organisation is similar
All prokaryotes have a _______ surrounding the cell membrane except _______
cell wall, mycoplasma
Genetic material in prokaryotes is held how in the cell?
basically naked, not enveloped
Prokaryotes have what all types of DNA?
- Genomic (single chromosome / circular DNA)
2. Plasmid (small circular DNA outside the genomic)
Plasmid DNA confers _______ to bacteria such as ________
unique phenotypic characters, antibiotic resistance
Plasmid DNA is present in _____________ (all / some / none) bacteria and _____________ (all / some / none) eukaryotes
some, none
Bacterial DNA is aka _____________ or _____________
nucleoid, main genomic DNA
_____________ in bacteria contains the essential genes whereas _____________ contains non-essential genes
Nucleoid, plasmid
Plasmid vs genomic DNA. Similarities (2) and differences (3,2)
Similarities - double-stranded, circular
Differences -
Plasmid is more than one, extrachromosomal and self-replicating independent of nucleoid
Genomic DNA is singular and naked
Bacterial surface structures are aka cell membrane extensions. T or F?
False, cell WALL extensions
A specialized differentiated form of cell membrane called _________ is found in prokaryotes. They are essentially _____ of cell membrane.
mesosome, infoldings
Most prokaryotic cells, particularly bacterial cells, have a chemically simple cell envelope. T or F?
False, complex
Cell envelope of prokaryotes consists of tightly bound 3 layered structure. What are the layers?
(outermost) Glycocalyx > Cell wall > Plasma membrane
Each layer of prokaryotic cell envelope has distinct features and functions. They do not have any coordinated function. T or F?
False, they have distinct functions but act together as a single protective unit
Bacteria can be classified into how many groups on basis of cell envelope differences? What are they? How are they identified?
2 groups,
Gram positive and Gram negative
Gram positive take up the gram stain, negative don’t
Glycocalyx differs in ______ and _____ among different bacteria
composition and thickness
Glycocalyx as a loose sheath is called _____ and as a thick and tough layer is called _______
slime layer;
capsule
Glycocalyx is made up of ___________
mucopolysaccharides (carbohydrates)
What are the functions of glycocalyx?
- protects from host immune system
- adhesion
- prevents loss of water and nutrients
- prevents from dessication
What are the functions of the cell wall layer of the cell envelope?
- Determines the shape of the cell
- Provides strong structural support
- Prevents bacterium from bursting or collapsing
Cell wall in bacteria is non-cellulosic. T or F?
True
Cell wall is made up of _____________ which in turn constitutes (3) things?
peptidoglycan
NAG - N-acetyl glucosamine
NAM - N-acetyl muramic acid
Amino acids / peptides
NAM and NAG alternate in cell wall of bacteria and peptide chains form on _________ (NAM / NAG)
NAM
What is cross-linking in bacteria?
Peptide bonds in cell wall form cross-links - grid-like structure - makes cell wall stronger
Penicillin prevents _____________ in bacteria thereby causing the cell wall to undergo _____________
cross-linking,
osmotic bursting
Why does penicilin not kill mycoplasma?
Because penicillin works by breaking down cell wall and mycoplasma has no cell wall
________ layer of prokaryotic cell envelope is selectively permeable and similar structurally to that of eukaryotes
Plasma membrane
Composition of cell wall in plants vs bacteria vs fungi?
Plants - cellulose
Bacteria - peptidoglycan
Fungi - chitin
Cell wall of plants, fungi and bacteria are made up of the same fundamental substance - carbohydrates. T or F?
True
_________ cells do not have cytoskeletal elements (threads on the inside providing structural support)
Bacteria or eukaryotic
Bacteria
Lysozyme in _______ and ______ in humans destroys bacteria by __________
saliva, tears, breaking down cell wall
Archaebacteria is not made up of peptidoglycan. T or F?
True
Gram positive bacteria retain the stain and are ______ in color.
Gram negative bacteria are colorless or ______ stain
blue, pink
Gram positive vs Gram negative bacteria
Comment on: cell wall/envelope - thin or thick?
amount of peptidoglycan and lipid?
presence of pili and porins
Gram positive - thick cell wall/envelope, more peptidoglycan but less lipid, no pilli or porins
Gram negative - thin cell wall/envelope, less peptidoglycan but more lipid, pilli and porins present
Categorize into Gram positive and Gram negative bacteria:
E Coli, Lactobaccillus, clostridium, mycoplasma, cyanobacteria, Frankia, Rhizobium, Streptococcus, Bacillus
E Coli - Negative Lactobaccillus - Positive Clostridium - Positive Mycoplasma - Negative Cyanobacteria - Negative Frankia - Positive Rhizobium - Negative Streptococcus - Positive Bacillus - Positive
Cell membrane is made up of a __________ bilayer
phospholipid
Cell membrane layer has a ______ head which is __________ (hydrophobic/hydrophillic) and two arms made up of _____ chains that are __________ (hydrophobic/hydrophillic)
polar, hydrophilic,
carbon, hydrophobic
Phospholipid in cell membrane is made up of _____ fatty acids and one ____________
two, phosphate
Mesosome is formed by extensions of cell wall or plasma membrane into the cell. T or F?
False, extensions of plasma membrane only
Mesosome plasma membrane extensions can be in what forms? (3)
vesicles
tubules
lamellae
What does mesosome help in? (6)
cell wall formation,
DNA replication and distribution to daughter cells,
respiration,
secretion processes,
increase surface area of plasma membrane,
increase enzymatic content
Mesosome is similar in function to _____________ in eukaryotes
cristae of mitochondria
What is a unique feature of the cyanobacteria cell envelope?
Chromatophores, membranous extensions into the cytoplasm containing pigments
Chromatophores are analogous to _____________ in eukaryotes
thylakoid of chloroplasts
Bacterial cells are always motile. T or F?
False, can be motile or non
Motile bacterial cells have ______ extensions from the cell wall called flagella
filamentous
Non-motile bacteria do not show movement. T or F?
False, they move passively (e.g. carried by water)
All bacteria have the same or similar flagella number and arrangement. T or F?
False, diverse
What are the 3 parts of a bacterial flagellum?
- Filament
- Hook
- Basal body
_________ is the longest portion of the bacterial flagellum. It extends from where to where?
Filament,
from cell surface to outside
Flagella of bacteria is made of single thread of _____________
flagellan protein
Filament is the largest surface structure in bacteria. T or F?
False, flagella
Bacterial and eukaroytic flagella differ in their roles and structural make-up. T or F?
False, differ in structural make-up but SAME role of movement
Bacterial flagella vs eukaryotic flagella? (2)
protein and arrangement
bacteria - flagellan protein, NO 9+2 arrangement
eukaryote - tubulin protein, 9+2 arrangement
What are the 6 types of flagella arrangement?
Monotrichous, Amphitrichous, Peritrichous, Cephalotrichous, Atrichous
Peritrichous has flagella _____________ for example _____________
in all directions, E. Coli
Cephalotrichous has flagella _____________ whereas lophotrichous has flagella _____________ (example: _____________)
on head, two ends (spirillium)
Atrichous bacteria are _____________ (with/without) flagella and _____________ (motile/non-motile)
Examples? (3)
without, non-motile
cyanobacteria, mycoplasma, coccus
Flagella, Pili and Fimbriae are 3 surface structures that play a role in bacterial motility. T or F?
False, 3 surface structures but latter 2 don’t play role in motility
Pilli are _________ and ________ surface structures made up of a special protein called _______
elongated, tubular, pillin
Fimbriae are _____________, ________-like fibres sprouting out of the bacterial cell
small, bristle-like
Role of pilli vs fimbrae
Pilli - sexual reproduction / conjugation
Fimbrae - attach to substratum (host or rock)
Fimbrae are less in number compared to pilli which are many in number. T or F?
False, other way around
Eukaryotic cells are usually measured in micrometers and some in millimeters. T or F?
True
Prokaryotic cells are usually measured in _________meters
micro
Size of ribosomes (in nm) in prokaryotic cells?
15 nm by 20 nm
What are the two subunits of ribosomes? Together they form?
50S and 30S, together form 70S prokaryotic ribosomes
50S is the smaller unit of ribosome whereas 30S is the larger unit. T or F?
False, reverse
Ribosomes are the site of ________
protein synthesis
Ribosomes are _____________ (membrane-bound or non-membrane bound)
non-membrane bound
What is a polysome? aka?
Several ribosomes attach to a single mRNA and form a chain, aka polyribosomes
Ribosomes translate mRNA into _____________ and once folded they become proteins
polypeptides
First ___S ribosomal subunit attaches to mRNA followed by ___S
30S, 50S
Inclusion bodies are soluble small-size organelles that are granular in appearance. T or F?
False, insoluble
Rest is correct
Why are inclusion bodies insoluble?
So that they take less space in cytoplasm
What are inclusion bodies?
reserve material in stored in prokaryotic cytoplasm
Inclusion bodies are not bound by any membrane and lie free in prokaryotic cytoplasm. T or F?
True
Examples of inclusion bodies?
Glycogen granules, phosphate granules, cyanophycean granules, glycogen granules
________ (type of inclusion body) are found in blue green and purple and green photosynthetic bacteria
Gas vacuoles
_____________ are aka pseudovacuoles. Why?
Gas vacuoles, they have a membrane (whereas other vacuoles don’t) but the membrane is NOT made up of lipid
Gas vacuoles are a type of _____________ (name of the organelle)
inclusion body
Gas. vacuoles are found in plant cells. T or F?
False, cyanobacteria