XI Chap 13 Photosynthesis Higher Plants Flashcards
What is photosynthesis?
physico-chemical process;
plants use light energy;
synthesis of organic compounds
Photosynthesis is important for what 2 reasons?
- primary source of all food on earth
2. oxygen into the atmosphere
__________ in ______ performed a series of experiments that revealed the role of air in the growth of green plants
Joseph Priestley in 1770
_______ discovered oxygen in _____
Joseph Priestley in 1774
________ used the same setup used in Priestley but modified it placing it once in dark and once in sunlight to show that ________
Jan Ingenhousz, sunlight is essential to plant process that purifies air
________ in an experiment with an aquatic plant showed that in bright sunlight, small ____ were formed around green parts of plants which did not form in dark
Jan Ingenhouse, bubbles of oxygen
It wasn’t until ____ that _______ provided evidence for production of glucose when plants grow.
1854, Julius von Sachs
What were the experiments done by TW Engelmann?
Described the action spectrum of photosynthesis.
Split light using a prism -> placed a green alga Cladophora in suspension of aerobic bacteria -> observed that bacteria accumulated in blue and red light
In what years was T W Engelmann alive?
1843 - 1909
By the middle of _______ century, key features of plant photosynthesis were known (i.e plants use light to make carbohydrates from CO2 and water).
19th
What was microbiologist Cornelius van Niel’s contribution to study of photosynthesis?
studies of purple and green bacteria,
showed that hydrogen from a suitable oxidisable compound reduces CO2 to carbohydrates
In green plants ______ is the hydrogen donor oxidized to O2
H2O
Some organisms do not release O2 during photosynthesis. T or F?
True
When H2S is the hydrogen donor for purple and green bacteria, the oxidation product is _____ or _____
sulphur or sulphate
How do we know that O2 evolved by green plants comes from H2O and not CO2?
Experiments with H2S and purple/green bacteria that produced sulphur/sulphate and not oxygen (later proved by using radioisotopic techniques)
What is the equation that represents photosynthesis accurately?
6CO2 + 12H2O —– (light) —-> C6H12O6 + 6H2O + 6O2
Photosynthesis is a single-step process represented by one equation. T or F?
False, multi-step process
________ cells in the leaves have a large number of chloroplast
Mesophyll
Why do chloroplasts align themselves to the walls of the mesophyll cells?
they get optimum quantity of incident light
There is clear division of labour in chloroplast. T or F?
True
Membrane system is responsible for trapping light energy in chloroplasts and for the synthesis of ATP and NADPH. T or F?
True
In stroma, enzymatic reactions synthesize ______ which in turn forms _____
sugar, starch
What are light and dark reactions?
Light - directly light driven
Dark - not directly light-driven but depend on products of light reactions
Light reactions are aka _________, dark reactions are aka _________
photochemical reactions,
carbon reactions / biosynthetic phase
Synthesis of ATP and NADPH - is it a light or dark reaction?
Light
Synthesis of sugar and starch - light or dark reactions?
Dark
Dark reactions occur in darkness and are not light-dependent. T or F?
False
You can separate leaf pigments of any green plant through ________ process
paper chromatography
Color in leaves are due to how many pigments? What are they and their colors on chromatogram?
4
Chlorophyll a - bright or blue green
Chlorophyll b - yellow green
Xanthophylls - yellow
Carotenoids - yellow to yellow orange
_______ are substances that have the ability to absorb light at specific wavelengths.
Pigments
_________ is the chief pigment associated with photosynthesis
Chlorophyll a
Most of photosynthesis takes place in ___ and ____ regions of the spectrum
blue and red
No photosynthesis takes place outside the blue and red regions of the spectrum. T or F?
False, some does
What are the pigments responsible for trapping light other than chlorophyll?
Thylakoid / accessory pigments: chlorophyll b, xanthophylls, cartenoids
Accessory pigments transfer the energy from light absorbed to chlorophyll a. T or F?
True
Accessory pigments enable a wider range of wavelength of incoming light to be utilized and protect chlorophyll a from ________
photo-oxidation
What is the photochemical phase?
Light reactions
What does the photochemical phase include?
Light absorption,
water splitting,
oxygen release and
formation of ATP and NADPH
What are the protein complexes involved in photochemical phase?
2 discrete photochemical light harvesting complexes, one in Photosystem I (PS I) and one in Photosystem II (PS II)
PS I and II are numbered according to the order….?
in which they were discovered NOT in which they function
What are LHC made up of?
hundreds of pigment molecules bound to proteins
Each photosystem has all the pigments except ____________
one molecule of chlorophyll a
What are antenna
light harvesting system formed by all the pigments in the photosystem
What role do LHC play?
make photosystem more efficient, pigments absorb different wavelengths of light
What is a reaction centre?
single chlorophyll a molecule, different in both photosystems
Absorption peaks for reaction centres for PS I and PS II?
PS I - absorption peak at 700nm (P700)
PS II - absorption peak at 680nm (P680)
In PS II, reaction centre absorbs 680nm wavelength of ____ light causing electrons to become excited and ________
red light,
jump into an orbit farther away from the atomic nucleus
Excited electrons in PS II are picked up by __________ which passes them on to an electrons transport
electron acceptor
Electron transfer system consists of ________
cytochromes
Movement of electrons in transport system is ________ (uphill or downhill) in terms of an oxidation-reduction or redox potential scale.
Downhill
Electrons are used up as they pass through the electron transport chain. T or F?
False, they’re passed on to pigments of PS I
Electrons in PS I are excited when they receive _____ light of wavelength _____ and are transferred to ______ that has _____ (lesser/greater) redox potential than PS II
red, 700nm, another acceptor molecule, greater
From the acceptor associated with PS I, electrons are moved _______ (uphill/downhill) to a molecule of _______
downhill,
energy-rich NADP+
Addition of electrons to NADP+ _____________ (oxidises/reduces) it to _____________
reduces
NADPH + H+
What is Z scheme?
Transfer of electrons starting from PS II - uphill to the acceptor - down the electron transport chain to PS I - excitation of electrons - transfer to another acceptor - finally down hill to NADP+ reducing it to NADPH + H+
Z-shape for Z-scheme is formed when all the carriers are placed in a sequence on __________ scale
redox potential scale
How does PS II supply electrons continuously?
Splitting of water, water is split into 2H+ , O and electrons
Splitting of water creates ______ one of the net products of photosynthesis
oxygen
Electrons needed to replace those removed from PS II are provided by PS I. T or F?
False, other way around. PS II supplies electrons to PS I
Water splitting complex is associated with PS I or II?
PS II
PS II is located on the inner side of the membrane of the ________
thylakoid
What is phosphorylation?
process by which ATP is synthesized by cells
Where does phosphorylation take place?
in mitochondria and chloroplasts
What is photo-phosphorylation?
synthesis of ATP from ADP and inorganic phosphate in presence of light
When PS II and PS I work in a series, ________ occurs. When only PS I is functional, ____________ occurs.
Non-cyclic photo-phosphorylation,
Cyclic photo-phosphorylation
Possible location for cyclic photophosphorylation is _______
stroma lamellae
Grana membrane or lamellae lacks PS II as well as NADP reductase enzyme. T or F?
False, stroma lamellae,
grana lamellae has both PS I and PS II
In cyclic photophosphorylation, excited electron does not pass on to _________ but is cycled back to the PSI complex through the ___________
NADP+,
electron transport chain
Cyclic flow results in the synthesis of _____
only ATP, not NADPH + H+
Cyclic photophosphorylation occurs when only light of ________ is available for excitation
wavelength beyond 680nm