Worksheet 1-3 Flashcards
important laboratory equipment in the study of living things
microscope
Four important attributes of the microscope
magnification
resolution
contrast
microscopic measurements
HPF equation
LPO magnification/HPO magnification *100
used to calibrate the ocular micrometer of the microscope
stage micrometer
1 ocular micrometer space equation
number of microns measured on the stage micrometer/number of ocular micrometer spaces
measured by its ability to differentiate two points in an object
resolving power
is a lens that stays in focus when magnification/focal length is changed
parfocal
microscope’s ability to enlarge an image of an object
magnification
refers to the darkness of the background relative to the specimen
contrast
is used to magnify an image of an object
lens
microscope can be classified as
mechanical/supporting
magnifying
illuminating
classification of the microscope used to support and adjust the parts
mechanical/supporting
classification of the microscope used to enlarge the specimen
magnifying
classification of the microscope that is not only used to provide light but for the observer to be able to see a clear image of the object being magnified
illuminating
micro means
small
skopos means
to look at
smallest the naked eye can see
100 micrometers/0.1mm
most human cell size
10 to 15 micrometers
ability to enlarge an image
magnification
magnification equation
image size / actual size
image size equation
magnificaton * actual size
ability to distinguish two close objects as distinct, shortest distance between two points
resolving power
resolving power equation
0.61(0.55)/numerical aperture
maximum resolution of a light microscope
200nm
maximum resolution of an electron microscope
0.5nm
typically 10x, but also come in 5x, 15x, and 20x varieties
eyepiece
also known as ocular lenses
eyepiece
is adjustable so that you can keep both eyes open when looking into the microscope
interocular distance
also called a revolving nose-piece or turret, this structure holds the objective lenses. Rotating them allows the objective lens to be switched.
nosepiece
lens closes to the object being viewed.
objective lenses
scanning lens magnifying power
4x