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Histopathology > STAINS FOR PROTEINS, ENZYMES AND NUCLEIC ACID > Flashcards

STAINS FOR PROTEINS, ENZYMES AND NUCLEIC ACID Flashcards

1
Q

Made up of amino acids linked by peptide bonds

A

Proteins

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2
Q

Fixative for amino acid histochemistry

A

Neutral Buffered Formol Saline

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3
Q

Detection of biological catalysts

A

Enzyme Histochemistry

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4
Q

Tissue fixed in acetone or formalin (-70°C or below) or 4% formalin overnight

A

Tissues for Enzyme Histochemistry

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5
Q

Technique for enzyme demonstration

A

Metal Precipitation

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6
Q

Composed of sugar, phosphate, and nitrogenous base

A

Nucleic Acids

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7
Q

Combined with basic proteins to form nucleoproteins

A

DNA and RNA

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8
Q

Histochemical ID of proteins; stains green

A

Alkaline Fast Green

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9
Q

Histochemical ID of cysteine and cystine; stains blue-green

A

Peracetic Acid-Alcian Blue

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10
Q

Histochemical ID of arginine; stains orange-red

A

Sakaguchi’s Arginine

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11
Q

Enzyme histochemistry; ALP staining; brownish black

A

Gomori Calcium

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12
Q

Enzyme histochemistry; ACP staining; black

A

Gomori Lead

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13
Q

Enzyme histochemistry; 5-nucleotidase; blackish brown

A

Lead Method

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14
Q

Enzyme histochemistry; ATPase staining; light to dark brown

A

Metal Precipitation ATPase

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15
Q

Enzyme histochemistry; Non-specific esterase; reddish brown

A

Alpha Naphthyl Acetate

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16
Q

Enzyme histochemistry; Non-specific esterase; blue

A

Indoxyl Acetate Method

17
Q

Enzyme histochemistry; dark brown to black

A

Acetylthiocholine Method

18
Q

Enzyme histochemistry; bluish black

A

Tetrazolium Method

19
Q

Enzyme histochemistry; black or bluish black

A

Succinic Dehydrogenase Method

20
Q

Nuclear DNA; red-purple; most reliable technique for DNA

A

Fuelgen Technique

21
Q

Differential stain for DNA and RNA; DNA - green; RNA - red

A

Methyl Green Pyronin

22
Q

Fluorescent dyes emit light when excited by UV or visible light

A

Fluorescent Stains

23
Q

Fluorescent stain for DNA and RNA; apple green; widely used

A

Fluorescein

24
Q

Fluorescent stain for DNA and RNA; orange-red

A

Rhodamine

25
Q

Fluorescent stain for DNA and RNA; DNA - yellow-green; RNA - orange-red

A

Acridine Orange

26
Q

Fluorescent stain for DNA; yellow; alternative to Basic Fuchsin

A

Acriflavine

27
Q

Pairing of complementary base pairs using labeled probes; identifies nucleic acids

A

In-situ Hybridization

28
Q

Gene amplification technique; steps include denaturation, annealing, and elongation

A

PCR (Polymerase Chain Reaction)

Histopathology (30 decks)

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