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Histopathology > Mounting and Labelling > Flashcards

Mounting and Labelling Flashcards

1
Q

Process of using a medium and coverslip to handle, store, and protect the tissue section

A

Mounting

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2
Q

Ideal coverslip thickness for photomicrography

A

1.5 (180nm)

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3
Q

Coverslip thickness commonly used for routine purposes

A

1 (150nm)

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4
Q

Coverslip thickness #2 measuring

A

210nm

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5
Q

Common coverslip sizes

A

18x18mm, 22x22mm, 20x20mm, 24x24mm

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6
Q

Refractive index of an ideal mounting medium (close to that of glass)

A

1.532

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7
Q

Methods of mounting sections

A

Slide lowered onto coverslip and inverted; Coverslip lowered onto slide

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8
Q

Used to mount water-miscible preparations; prevents drying and cracking

A

Aqueous mounting media

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9
Q

Components of aqueous mounting media

A

Gelatin, glycerin jelly, gum arabic, glycerol, sugar, preservative (e.g., merthiolate)

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10
Q

Water-miscible media used for semi-permanent sections

A

Glycerin

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11
Q

Cheapest aqueous mounting medium

A

Water

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12
Q

Standard mounting medium for fat stains; refractive index: 1.46

A

Farrant’s Medium (Gum Arabic Medium)

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13
Q

Aqueous medium for methylene blue-stained nerve preparations; refractive index: 1.44

A

Apathy’s Medium

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14
Q

Used for mounting frozen sections from water

A

Brun’s Fluid

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15
Q

Long-term media for dehydrated and xylene-cleared preparations

A

Resinous mounting media

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16
Q

Transparent, colorless resin that darkens with age; refractive index: 1.524

A

Canada balsam

17
Q

Natural resin source of Canada balsam

A

Abus balsamea (Canadian fir tree)

18
Q

Resinous medium for small tissue sections; dries rapidly; refractive index: 1.42

A

DPX

19
Q

Synthetic resin dissolved in xylene

A

XAM

20
Q

Sealing method to prevent fluid evaporation and sticking of slides

A

Ringing

21
Q

Ringing medium made of paraffin and colophonium resin

A

Kronig Cement

22
Q

Ringing medium made of cellulose adhesives

A

Durofix/Du Noyer’s

23
Q

Slide labeling requirements

A

Year and specimen number for identification

Histopathology (30 decks)

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