METHODS OF TISSUE EXAMINATION

Question 1

Examined in a living state; Allows observation of protoplasmic activities (phagocytosis, mitosis, pinocytosis, movement); Not permanent

Answer 1

Fresh Tissues

Question 2

Teasing or Dissociation, Squash Preparation (Crushing), Smear Preparation

Answer 2

Methods of Fresh Tissue Examination

Question 3

Tissue immersed in NSS, dissected or separated, examined under the microscope (phase contrast or bright field), can be stained with methylene blue

Answer 3

Teasing or Dissociation Method

Question 4

Small tissues (1mm or less) placed on a slide, compressed with another slide or coverslip, supravital stains can be added

Answer 4

Squash Preparation (Crushing) Method

Question 5

Cellular materials spread lightly over a slide, useful for cytology

Answer 5

Smear Preparation Method

Question 6

Material added with an applicator stick or loop in a direct or zigzag fashion

Answer 6

Streaking Method

Question 7

Material placed on a clean slide and spread into moderately thick film by teasing with an applicator stick

Answer 7

Spreading Method

Question 8

Maintains intercellular relationships

Answer 8

Advantage of Spreading Method

Question 9

Use of 2 slides in preparing the smear for thick secretions

Answer 9

Pull-Apart Method

Question 10

Examined in a living state; Allows observation of protoplasmic activities (phagocytosis, mitosis, pinocytosis, movement); Not permanent

Answer 10

Fresh Tissues

Question 11

Teasing or Dissociation, Squash Preparation (Crushing), Smear Preparation

Answer 11

Methods of Fresh Tissue Examination

Question 12

Tissue immersed in NSS, dissected or separated, examined under the microscope (phase contrast or bright field), can be stained with methylene blue

Answer 12

Teasing or Dissociation Method

Question 13

Small tissues (1mm or less) placed on a slide, compressed with another slide or coverslip, supravital stains can be added

Answer 13

Squash Preparation (Crushing) Method

Question 14

Cellular materials spread lightly over a slide, useful for cytology

Answer 14

Smear Preparation Method

Question 15

Material added with an applicator stick or loop in a direct or zigzag fashion

Answer 15

Streaking Method

Question 16

Material placed on a clean slide and spread into moderately thick film by teasing with an applicator stick

Answer 16

Spreading Method

Question 17

Maintains intercellular relationships

Answer 17

Advantage of Spreading Method

Question 18

Use of 2 slides in preparing the smear for thick secretions

Answer 18

Pull-Apart Method

Question 19

Freshly cut tissue pressed onto a clean, sterile, polished slide to make an impression smear

Answer 19

Touch Preparation (Impression Smear)

Question 20

1st and most important step in histopathology; Identify specimens properly without needing patient name on the specimen tag; Enter details in a logbook; Specimen number preceded by S (surgical), A (autopsy), C (cytology); Year indicated

Answer 20

Numbering (Accessioning)

Question 21

Example of specimen numbering: S99-0345

Answer 21

(S for surgical, 99 for year, 0345 for entry number)

Question 22

After numbering, pathologist describes the specimen grossly; MT writes description on the back of the request with the use of

Answer 22

Pencil for writing the description

Question 23

Specimen size for processing

Answer 23

2x3 cm and 3-5 mm thick

Question 24

Can be added for small tissues

Answer 24

1% eosin

Question 25

Tissue slices should be taken at _____to the surface of the organ

Answer 25

Right angles

Question 26

For electron microscopy, specimen size should be

Answer 26

1mm^3