STAINING OF MICROORGANISMS

Question 1

Bacteria color: Gram (+) purple, Gram (-) red

Answer 1

Gram Stain

Question 2

Bacteria color: Gram (+) blue, Gram (-) red, counterstain replacing neutral red

Answer 2

Gram Twort

Question 3

Bacteria and tissue specimen stain, Gram (+) blue, Gram (-) red, used for Nocardia, Actinomyces

Answer 3

Brown and Brenn

Question 4

Acid-fast staining for mycobacteria, AFB red

Answer 4

Ziehl Neelsen

Question 5

Leprosy and Nocardia AFB red stain

Answer 5

Wade Fite

Question 6

Sensitive fluorescence for acid-fast mycobacteria, golden yellow

Answer 6

Auramine and Rhodamine

Question 7

Stain for Helicobacter, blue violet

Answer 7

Toluidine Blue

Question 8

Stain for Legionella and spirochetes, dark brown to black

Answer 8

Dieterile Method

Question 9

Stain for spirochetes, treponema, black

Answer 9

Levaditi’s

Question 10

Stain for spirochetes, Donovan bodies, fungi, black

Answer 10

Modified Steiner and Steiner

Question 11

Stain for spirochetes, black

Answer 11

Warthin Starry

Question 12

Best tissue stain for fungi, sharply outlined black

Answer 12

Grocott Methenamine Silver

Question 13

Stain for hyphal elements, yeast, magenta

Answer 13

PAS

Question 14

Stain for viral inclusions, bright red

Answer 14

Lendrum’s Phloxine Tartrazine

Question 15

Stain for HBs Ag, brown-black

Answer 15

Orcein

Question 16

Stain for blood and marrow parasites, toxoplasma, spirochetes

Answer 16

Giemsa

Question 17

General stain for electron microscopy, heavy metal

Answer 17

Phosphotungstic

Question 18

Superior stain for electron microscopy, heavy metal

Answer 18

Uranyl Acetate

Question 19

Primary and secondary stain for electron microscopy, heavy metal

Answer 19

Lead

Question 20

Precaution to remove stains from skin

Answer 20

Acid Alcohol (ADD)