Stains for CNS

Question 1

Fixative commonly used for neuron and nerve fiber staining

Answer 1

Formol Saline, Buffered Formalin

Question 2

Stain for neurons, axons, and neurofibrils with a grayish background

Answer 2

Bicelschowsky’s

Question 3

Stain for nerve fibers, nerve endings, and neuritic plaques for Alzheimer’s diagnosis

Answer 3

Bodian’s

Question 4

Stain used to identify neuritic plaques and neurofibrillary tangles for Alzheimer’s

Answer 4

Sevier Munger

Question 5

Stain for Nissl granules (RNA) with purple or dark blue color

Answer 5

Cresyl Fast Violet

Question 6

Other stains for Nissl bodies besides Cresyl Fast Violet

Answer 6

Toluidine Blue, Methylene Blue, Thionine, H and E

Question 7

Stain for myelin sheath with blue-black color, but time-consuming

Answer 7

Weigert Pal

Question 8

Stain for myelin with blue-green color, easier than Weigert Pal, and includes counterstain for Nissl granules

Answer 8

Kluver and Barrera Luxol Fast Blue

Question 9

Stain for myelin with blue-green color, good for frozen sections

Answer 9

Luxol Fast Blue

Question 10

Stain for reactive astrocytes

Answer 10

Modified PTAH Stain

Question 11

Stain for astrocytic processes

Answer 11

Modified Holzer’s

Question 12

Immunohistochemistry for normal oligodendrocytes

Answer 12

Ab to Galactocerebroside, Myelin Basic Protein, Carbonic Anhydrase C

Question 13

Immunocytochemistry for microglia

Answer 13

CD68

Question 14

Stain used for Negri bodies, appearing deep magenta

Answer 14

Schieifsteins