CONNECTIVE TISSUE STAINING

Question 1

Cells responsible for producing connective tissue fibers

Answer 1

Fibroblast

Question 2

Most abundant connective tissue fiber type

Answer 2

Collagen

Question 3

Connective tissue fiber type responsible for stretch and recoil

Answer 3

Elastic

Question 4

Connective tissue fiber type characterized by branching and being silver-loving (argyophilic)

Answer 4

Reticular

Question 5

Stains reticulin fibers black using silver impregnation

Answer 5

Gomori’s Silver Impregnation

Question 6

Stains collagen pink/deep red, simplest method for collagen

Answer 6

Van Gieson’s

Question 7

Stains collagen blue in connective tissue

Answer 7

Masson’s Trichrome

Question 8

Demonstrates various connective tissue fibers, not specific for collagen, uses colorful method

Answer 8

Mallory’s Aniline Blue

Question 9

Stains amyloid, connective tissue, and mucus colloid deep blue, showing minute details

Answer 9

Azocarmine Stain

Question 10

Stains elastic fibers dark blue or blue-black

Answer 10

Weigert’s

Question 11

Stains elastic fibers black, rapid method

Answer 11

Verhoeff’s

Question 12

Stains elastic fibers dark brown using Taenzer Unna method

Answer 12

Orcein

Question 13

Bright red stain for elastic fibers and dark blue for fibrin and connective tissue

Answer 13

Krajian’s

Question 14

Insoluble fibrillar protein seen after tissue damage or acute inflammatory reactions

Answer 14

Fibrin

Question 15

Eosinophilic material with staining reactions identical to fibrin, seen in collagen diseases or hypersensitivity

Answer 15

Fibrinoid

Question 16

Pathologic exudates and deposits stained nonspecifically by PAS

Answer 16

Hyalin

Question 17

Semi-translucent ground glass or hyaline eosinophilic substance found in CT cells, kidneys, spleen, adrenals

Answer 17

Amyloid

Question 18

Stains fibrin red and blue in the Martius Scarlet Blue technique

Answer 18

MSB Technique (Martius Scarlet | Fibrin Red Blue)

Question 19

Stains fibrin dark blue with hematoxylin in the Mallory’s technique

Answer 19

Mallory’s Phosphorungstic Acid

Question 20

Stains amyloid with delicate purple or blue color

Answer 20

Gram’s Iodine

Question 21

Stains amyloid red, alkaline method of choice

Answer 21

Congo Red

Question 22

Stains amyloid purplish red with metachromatic staining, not the recommended method

Answer 22

Methyl Violet Crystal Violet

Question 23

Fluorescent stain for amyloid with silver blue/yellow fluorescence

Answer 23

Thioflavine