embedding

Question 1

Done after wax impregnation

Answer 1

Embedding

Question 2

Positioning the tissue in the mold; generally the surface of the section to be cut should be parallel to the bottom of the mold

Answer 2

Orientation

Question 3

Tubular structures (arteries, fallopian tubes) cut in

Answer 3

x-section of the lumen

Question 4

Skin, intestine, gall bladder, and other epithelial biopsies cut in

Answer 4

a plane at right angles to the surface

Question 5

Muscle biopsies cut in

Answer 5

transverse longitudinal planes

Question 6

2 L-shaped strips of heavy brass or metal; size of mold is adjustable

Answer 6

Leuckhart’s embedding mold

Question 7

multiple compartments or grids for multiple embedding

Answer 7

Compound embedding unit

Question 8

stainless steel base mold with plastic embedding ring, e.g., Tissue Tek

Answer 8

Plastic embedding rings and base molds

Question 9

peel away (3 sizes; 22x22mm, 22x30mm, 22x40mm), ice tray, paper boat (no need for trimming)

Answer 9

Disposable molds

Question 10

used for fragmentary biopsies

Answer 10

Watch glasses

Question 11

Tissue processing and embedding system composed of 3 pieces: perforated bottom, frame center piece, and perforating lid

Answer 11

TIMS

Question 12

Advantage of TIMS

Answer 12

fixation, dehydration, clearing, and embedding

Question 13

Embedding medium used for hard tissues and large sections of whole organs

Answer 13

Celloidin or Nitrocellulose method

Question 14

Double infiltration technique: first with celloidin, then embedded with paraffin

Answer 14

Double embedding method

Question 15

Embedding technique for electron microscopy to allow tissue sectioning of 80nm thickness

Answer 15

Plastic resin embedding

Question 16

Embedding method used for high-resolution light microscopy and renal biopsies (tissue section of 2mm)

Answer 16

Plastic resin embedding

Question 17

Embedding method suitable for extremely hard tissues

Answer 17

Plastic resin embedding

Question 18

Embedding medium with the widest application for both light and electron microscopy, capable of producing sections of 30-40nm thickness, preferred for TEM

Answer 18

Epoxy

Question 19

Most stable epoxy resin, slow acting, used for electron microscopy embedding

Answer 19

Bisphenol A (Araldite)

Question 20

Epoxy resin, less stable but useful, commonly used for embedding

Answer 20

Glycerol (EPON)

Question 21

Epoxy resin that infiltrates the fastest but is highly toxic

Answer 21

Cyclohexene dioxide (Spurr)

Question 22

Seldom used, applied in electron microscopy

Answer 22

Polyester

Question 23

Preferred embedding medium for high-resolution light microscopy

Answer 23

Acrylic plastics

Question 24

Ideal embedding medium for undecalcified bone, an example of methacrylate

Answer 24

Methyl methacrylate (acrylates)

Question 25

Used for TEM, involves the use of polyglycol methacrylate and glycerol methacrylate

Answer 25

Methacrylates