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biotech > principe hybridisatie > Flashcards

principe hybridisatie Flashcards

1
Q

hybridisatie

A

proces waarbij gemerkte DNA-moleculen in oplossing (probes) gaan baseparen met comp DNA/RNA geïmmobiliseerd of ook in oplossing of op coupe

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2
Q

Tm

A

smelttemperatuur
bepaalt denaturatie & renaturatie
1/2 H-bruggen verbroken, 1/2 doelseq gebonden met probe
condities renaturatie bepaalt door:
temp, pH, zoutconc, formamide conc

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3
Q

homoduplexen

A

perfecte baseparing (hoge stringentie & formamide, lage zoutconc)

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4
Q

heteroduplexen

A

1 of meerdere basen niet gepaard (lage stringentie)

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5
Q

soorten

A

filterhybridisatie
oplossing-hybridisatie
in situ hybridisatie
reverse hybridisatie

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biotech (48 decks)

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