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biotech > expressie gisten > Flashcards

expressie gisten Flashcards

1
Q

voordelen

A

goedkoop
gem. schaal vergroten
splicing
PTM

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2
Q

nadelen

A

splicing niet zoals hogere euk
PTM niet zoals hogere euk

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3
Q

geschikte vectoren

A

klein
unieke knipplaatsen
shuttle plasmide
selectiemerker E.coli (AB) & gist (TRP1,URA3,LEU2,HIS3)

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4
Q

vectoren genexpressie

A

YEp (yeast episomal plasmid) naast genoom> gist Z p
YCp (yeast centromere vector)> zelf centromeer
YIp (yeast integrating plasmid) > stabiele int

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biotech (48 decks)

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