andere methoden genetische variatie bestuderen Flashcards

1
Q

tandem repeats

A

> overeenstemmingen in kaart
- minisatelieten = VNTR = variable number tandem repeats (gekend)
- weefsel speciiek tot expressie

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2
Q

(finger)typing

A

primer rond VNTR
fingerprint = genotypische identificatie individu bep soort
meerdere loci via multiplex
» AFLP-PCR of RAPD-PCR

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3
Q

TP-PCR tekening

A

p86

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4
Q

exon-PCR + sequenering

A

PCR amp alle exonen + GEF + seq alle exonen
detectie mutatie software-analyse

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5
Q

HRM + tekening

A

= gerichter
exon-PCR staal vs referentiegenoom WT
heteroduplexen, smelten & reannealen
- saturerende kleurstof!

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6
Q

feno vs geno

A

tijdens meiose I & crossing over

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