PARA LAB LA3

Question 1

can improve the sensitivity of detection of parasites

Answer 1

Stool concentration techniques

Question 2

____________________________ do not usually survive the concentration procedure

Answer 2

Protozoan Trophozoites

Question 3

concentration techniques can separate ___________ and _______________ from a larger amount of stool

Answer 3

Protozoan cysts and Helminth Eggs

Question 4

parasites have a higher specific gravity than the reagent, therefore the parasite will sink to the bottom of the preparation

Answer 4

Sedimentation Technique

Question 5

the parasite has a lower specific gravity and will therefore float to the surface of the preparation.

Answer 5

Flotation Technique

Question 6

Mounts prepared from __________________ are much cleaner than those from __________________________

Answer 6

flotation techniques, sedimentation preparation

Question 7

the most widely used sedimentation technique

Answer 7

FECT

Question 8

technique useful for the recovery of helminth eggs and protozoan cysts

Answer 8

FECT

Question 9

claimed to be more efficient than ether in the recovery of cestode eggs and Giardia eggs

Answer 9

Ethyl Acetate

Question 10

how much stool is used for FECT

Answer 10

1-1.5g

Question 11

layers found in FECT

Answer 11

ETHER
DEBRIS (fatty debris)
FORMALIN
SEDIMENT

Question 12

technique recommended for the recovery of Trichuris, Capillaria, and trematode eggs, especially schistosoma

Answer 12

AECT

Question 13

amount of stool used for AECT

Answer 13

1g

Question 14

widely used for Schistosomiasis Control and Research Project in the Philippines

Answer 14

MIF CT

Question 15

technique found efficient in the qualitative diagnosis of Schistosomiasis

Answer 15

MIF CT

Question 16

can be used for staining protozoan cysts and trophozoites in wet mount preparation and as a preservative for these stages and for helminth eggs and larvae

Answer 16

MIF solution

Question 17

how to prepare the stain solution of the MIF

Answer 17

8.4ml of stock MF into a test tube containing 0.6ml of 5% Lugol’s solution

Question 18

main reagent for zinc sulfate flotation technique

Answer 18

33% zinc sulfate solution

Question 19

if parasites are exposed to high specific gravity, distortion and shrinkage of __________________ and ____________________ may occur

Answer 19

protozoan cysts, and nematode eggs

Question 20

disadvantage of Zinc Sulfate Flotation

Answer 20

some helminth eggs are very dense and will not float

Question 21

makes use of table salt solution

Answer 21

Brine Flotation Technique

Question 22

Brine Flotation Technique is prepared by ________________________

Answer 22

dissolving 40g of NaCl or salt on 100ml of water to give a specific gravity between 1.18-1.20

Question 23

disadvantage of Brine Flotation

Answer 23

helminth eggs like hookworm and Schistosoma become fully shrunken

Question 24

Brine Flotation Technique is not useful for _________________________ because they have specific gravity higher than 1.20

Answer 24

Operculated Eggs

Question 25

boiled sugar solution preserved with phenol is used in this method

Answer 25

Sheather’s sugar Flotation Technique

Question 26

this technique is considered the best for the recovery of coccidian oocytes

Answer 26

Sheather’s sugar Flotation Technique

Question 27

Sheather’s sugar Flotation Technique visualization of oocytes can be better appreciated through the use of a _______________________________

Answer 27

phase contrast microscope