BACTE LAB LA5

Question 1

describes a process that destroys or eliminates all forms of
microbial life and is carried out in health-care facilities by physical or
chemical methods.

Answer 1

STERILIZATION

Question 2

describes a process that eliminates many or all pathogenic
microorganisms, except bacterial spores, on inanimate objects. In
health-care settings, objects usually are disinfected by liquid chemicals or
wet pasteurization. Each of the various factors that affect the efficacy of
disinfection can nullify or limit the efficacy of the process.

Answer 2

DISINFECTION

Question 3

A process of washing, cleaning, or removing dirt, and eliminates dust, debris, and germs on the surface. It doesn’t kill germs, but by removing them, it lowers their numbers and risk of infection

Answer 3

SANITIZING

Question 4

a process of destroying all forms of microbial life and is carried out in medical facilities by physical or chemical methods

Answer 4

STERILIZING

Question 5

using chemicals to kill germs on surfaces. This process doesn’t necessarily clean dirty surfaces or remove germs, but killing forms on a surface after cleaning, can further lower the risk of opening

Answer 5

DISINFECTING

Question 6

agent used to disinfect inanimate objects or surfaces but is generally toxic
to use on human tissues

Answer 6

Disinfectant

Question 7

gent that kills or inhibits growth of microbes but is safe to use on human
tissue.

Answer 7

Antiseptics

Question 8

agent that reduces, but may not eliminate, microbial numbers to a safe level.

Answer 8

Sanitizer

Question 9

something (such as an antiseptic or disinfectant) that destroys or inactivates
pathogens (such as bacteria, viruses, and fungi)

Answer 9

Germicide

Question 10

PHYSICAL METHODS OF STERILIZATION

Answer 10

Heat Sterilization, filtration, irradiation

Question 11

CHEMICAL METHODS OF STERILIZATION

Answer 11

Gaseous Sterilization (Ethylene oxide, Formaldehyde, Nitrogen dioxide, Ozone

Liquid Sterilization
-Hydrogen peroxide
- Glutaraldehyde
- Hypochlorite

Question 12

Most EPA-registered hospital
disinfectants have a label contact time of ________________________. However, many
scientific studies have demonstrated
the efficacy of hospital disinfectants
against pathogens with a contact time of at least _________________

Answer 12

10 minutes, 1 minute.

Question 13

Microbial communities that are tightly attached to surfaces
and cannot be easily removed

Answer 13

Biofilm

Question 14

Factors affecting the efficacy of disinfection and sterilization

Answer 14

• Number and Location of Microorganisms
• Innate Resistance of Microorganisms
• Concentration and Potency of Disinfectants
• Physical and Chemical Factors
• Organic and Inorganic matter
• Duration of exposure
• Biofilms