BACTE LAB LA1 Flashcards
Is a framework that describes the use of specific practices,
training, safety equipment, and specially designed buildings to protect the
worker, community, and environment from an accidental exposure or
unintentional release of infectious agents and toxins
BIOSAFETY
include biological agents and toxins infectious to humans,
animals, wildlife, or plants such as parasites, viruses, bacteria, fungi, prions; and
biologically-active materials such as toxins, allergens, and venoms. In addition to
causing disease in living organisms, biohazards can cause significant damage to
the environment.
BIOHAZARD
Designed to protect the operator, the laboratory environment and work materials from exposure to infectious aerosols and splashes when manipulating materials containing infectious agents.
BIOSAFETY CABINET
traps 99.97% of particles with 0.3 um in diameter and 99.99% of particles with greater size
HEPA FILTERS
HEPA meaning
High Efficiency Particulate Air
Advantage: provides personnel and environmental protection
CLASS 1 BSC
Used for: radionuclides and volatile toxic chemicals
CLASS 1 BSC
Does not provide reliable product protection
CLASS 1 BSC
Advantage: provides personnel and work surface materials from contaminated room air
CLASS 2 BSC
Allows only air from a HEPA-filtered (sterile) supply to flow over the work surface
CLASS 2 BSC
Types of CLASS II BSC
A1, A2, B1, B2
Used for: Risk group 2 and 3
CLASS 2 BSC
Advantage: provides the highest level of personnel protection
CLASS 3 BSC
Used for: Risk 4
CLASS 3 BSC
Access to the work surface is by means of heavy duty rubber gloves. Also have an attached pass-through box that can be sterilized and is equipped with a HEPA-filtered exhaust
CLASS 3 BSC
describes the use of biosafety principles and practices
in laboratories to reduce the health-related risks associated with handling
infectious agents, toxins and other biological hazards arising from an accidental
exposure or unintentional release
LABORATORY BIOSAFETY
are designations applied to projects or activities
conducted in laboratories in ascending order of containment based on the
severity of the health-related risk associated with the work being conducted
BIOSAFETY LEVELS
The appropriate BSL to be assigned to a project is determined by _______________ or professionals, and reflects the specific
combinations of specially designed buildings, safety equipment and safe work
practices that laboratory workers must use
institutional biosafety committees (IBCs)
refers to the use of safety equipment and
specially designed rooms and buildings which act as primary barriers to prevent
the release of infectious agents, toxins, and other biological hazards into the
environment
LABORATORY BIOCONTAINMENT
Examples of Primary biocontainment barriers include, but are not limited to:
o biosafety cabinets
o sealed containers such as containment centrifuges
o special animal caging systems
Include specially designed and
constructed rooms and buildings. These buildings include several engineering features to keep, or “contain”, infectious agents, toxins, and biological hazards
inside the laboratory workspace
Secondary biocontainment barriers
Secondary biocontainment barriers features include, but are
not limited to:
o sealed rooms;
o self-closing, lockable doors; and
o specialized air handling and decontamination systems
is a term used to describe laboratories or facilities that
operate at Biosafety Level 3 (BSL-3) conditions
High containment
is a term used to describe laboratories or facilities that operate at BSL-4 conditions.
“Maximum containment”
is a combination of the likelihood of an exposure to an infectious
agent, toxin, or biological hazard that will cause harm, and the consequence,
or severity, of that harm if exposure does occur.
Biorisk
is a process that evaluates multiple factors to
determine the risk to laboratory workers, the community, or the environment of
working with an infectious agent, toxin, or other biological hazard.
biological risk assessment
used to determine the appropriate biosafety level for each project conducted within a laboratory
biological risk assessment
are classifications of infectious
agents and toxins that identify the relative likelihood and severity of disease an
infectious agent or toxin can cause.
Risk groups
organisms that are not associated with disease in
healthy human adults
RISK GROUP 1
organisms that may cause mild disease for which
medical countermeasures are available.
RISK GROUP 2
organisms that may cause serious or deadly disease for
which medical countermeasures may be available, and which have
low potential to spread in the community or environment
RISK GROUP 3
organisms that will cause serious or deadly disease for
which medical countermeasures are unlikely to be available, and
which have high potential to spread in the community or
environment
RISK GROUP 4
