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2A PRELIM > BACTE LAB LA2 > Flashcards

BACTE LAB LA2 Flashcards

1
Q

deals with detecting, culturing, and identifying microorganisms like bacteria, fungi, viruses, etc.

A

MICROBIOLOGY LABORATORY

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2
Q

Example of Obligate Anaerobes

A
  • Clostridium
  • Bacteroides
  • Fusobacterium
  • Pepto streptococcus
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3
Q

also known as tissue culture hoods, are
designed to provide personnel, environmental and product protection when
appropriate practices and procedures are followed

A

Biological Safety Cabinets

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4
Q

BSC certification validity

A

12 months

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5
Q

Operate the BSC _________ for at least ______________ before beginning work to
allow the BSC to “purge” particulates

A

blowers, 3-5 minutes

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6
Q

Disinfect all interior surfaces of BSC work area with an appropriate surface
disinfectant which include:

A

10% bleach followed by 70% ethanol

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7
Q

Perform all operations at least _____________ from the front grille on the work surface.

A

4 inches

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8
Q

is a spill that is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel

A

MINOR SPILL

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9
Q

In minor spill Allow disinfectant _______________ of contact time before wiping up the spill.

A

20 minutes

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10
Q

In minor spills Allow BSC to run an additional ___________ before resuming work or shutting down.

A

10 minutes

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11
Q

is a spill requires active assistance from emergency personnel.

A

MAJOR SPILL

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12
Q

are commonly used in healthcare settings to sterilize medical devices.

A

AUTOCLAVES

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13
Q

The items to be sterilized are placed inside an autoclaves’ pressure vessel or is commonly referred to as the ____________

A

chamber

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14
Q

How often is the checking for AUTOCLAVE timing

A

Quarterly

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15
Q

Three factors are critical to ensuring successful steam sterilization in an autoclave:

A

time, temperature and steam
quality

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16
Q

3 Phases of Autoclave Process

A

Conditioning Phase
Exposure Phase
Exhaust Phase

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17
Q

Air inhibits sterilization and must be removed from the chamber during the first phase of the sterilization cycle known as ______________________

A

Conditioning

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18
Q

use steam to displace the air in the chamber and force
the air down the sterilizer drain.

A

Gravity-type sterilizers

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19
Q

After the air is removed, the sterilizer drain closes and steam is continuously admitted into the chamber, rapidly increasing the pressure and temperature inside to a predetermined level. The cycle enters the ________________ and items are held at the sterilization temperature for a fixed amount of time required to sterilize them.

A

Exposure Phase

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20
Q

During the final phase of the cycle, the sterilizer drain is opened and steam is removed, depressurizing the vessel and allowing the items in the load to dry.

A

Exhaust Phase

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21
Q

are designed to validate that an autoclave run was successful at killing microorganisms

A

Biological indicator systems

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22
Q

__________________________ spores are highly resistant to steam and vaporized hydrogen peroxide and are therefore most commonly used in biological indicator tests.

A

Geobacillus stearothermophilus

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23
Q

are used to validate whether an autoclave is working properly.

A

Chemical and biological indicators

24
Q

are quick to read, but generally, only demonstrate that the autoclave has reached a certain minimum temperature.

A

Chemical indicators

25
Q

They do not give an indication of how long that high temperature was held or whether the procedure was sufficient to provide adequate sterilization.

A

Chemical indicators

26
Q

take more time to read but measure the
growth or inhibition of bacterial spores generally known to have high resistance
to steam and vaporized hydrogen peroxide.

A

Biological indicators

27
Q

G. stearothermophilus spores are
inactivated when exposed to temperatures of _______ for at least ____________

A

121.1oC, 20 minutes

28
Q

irreversibly change color after being exposed to normal autoclave operating temperatures of 121oC (250oF) at 15
psi.

A

Chemical Indicators

29
Q

can give you a quick visual reference for heat penetration inside the
load. They are frequently sold as paper or card strips that are positioned near
the center of each load, and toward the bottom front of the autoclave.

A

Chemical Indicators

30
Q

are not designed nor intended to prove that
organisms were actually killed during a decontamination cycle

A

Chemical Indicators

31
Q

Measures the precision in determining the mass of solid objects, liquids, powders, and granular substances.

A

Top Loading Balance

32
Q

Calibrate weight balance every ______

A

Monthly

33
Q

is used mainly in laboratories requiring moderately precise volume measurements . It is narrow and cylindrical with markings for volume measurement. Along with the volume of liquid, it is also indirectly used to measure the mass of a solid.

A

Graduated Cylinder

34
Q

Are containers made of glass, plastic, borosilicate or related substances, and topped by special caps or stoppers. They are intended to contain chemicals in liquid or powder form for laboratories and stored in cabinets or on shelves.

A

Media Bottle

35
Q

It helps grow microorganisms by providing an artificial environment, preparing stock cultures, and performing various biochemical tests.

A

standard test tube

36
Q

The microbiology laboratory uses test tubes with __________, and the open end is plain without lips

A

borosilicate glass

37
Q

provides a broader surface area for growing the organism than tubes and bottles.

A

Petridish

38
Q

Inventor of Petri Dish

A

German bacteriologist Julius Richard Petri

39
Q

designed for use with isopropyl alcohol, denatured ethyl alcohol, or methylated spirits.

A

ALCOHOL LAMP

40
Q

benchtop laboratory tools that are used to heat evenly and mix various types of liquids and solutions

A

Hotplates and hotplate stirrers

41
Q

hot plates that are used for heating only

A

Conventional Hot Plates

42
Q

able to simultaneously heat and mix.

A

combination hotplate stirrers

43
Q

is molded of silicone rubber to provide superior protection for handling hot or cold objects.

A

Hot Hand Protector

44
Q

HOTHANDS withstands temperatures up to _______________ or down to ____________________.

A

500°F (260°C), -70°F (-57°C)

45
Q

hand-held and compact appliances that introduce microorganisms like bacteria or yeast into plated or tubed growth media before incubation, multiplication, or growth

A

Inoculating loops and needles

46
Q

a possible substitute for sterilizing the loops and wire

A

bacterial incinerator

47
Q

is a laboratory tool used in histology and microbiology to hold and facilitate the staining of microscope slides

A

slide staining rack

48
Q

it helps highlight specific structures or components within the samples.

A

Staining

49
Q

is used to set the desired temperature of the
incubator

A

Thermostat

50
Q

This is the most common type of incubator used in most
of the laboratories. These incubators are the basic types of incubators with
temperature control and insulation.

A

Benchtop incubators

51
Q

incubators are the special kinds of incubators that are
provided with automatic control of CO2 and humidity

A

CO2 incubators

52
Q

CO2 incubators are used for the growth of the cultivation of different bacteria requiring ______ of CO2 concentration

A

5-10%

53
Q

For incubation at temperatures below the ambient,
incubators are fitted with modified refrigeration systems with heating and
cooling controls

A

Cooled incubator

54
Q

A thermostatically controlled shaker incubator is another
piece of apparatus used to cultivate microorganisms.

A

Shaker incubator

55
Q

s are smaller in size and are used in
fieldwork, e.g. environmental microbiology and water examination

A

Portable incubator -

2A PRELIM (16 decks)

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