Papillomaviridae and Polyomaviridae

Question 1

Papilloma virus genetics?

Answer 1

ds circular DNA
Icosahedral (L1+L2)
Non-enveloped

Question 2

What do the viral proteins of Papilloma virus do?

Answer 2

L1 – Major Caspid Protein (80%)

L2 – Minor Caspid (20%)

Question 3

Where in the body does Papilloma virus replication occur?

Answer 3

Differentiated Skin and Mucosa Epithelium

Question 4

Papilloma virus infects what species?

Answer 4

Only Humans

Question 5

Clinical Presentation of cutaneous Papilloma virus?

Answer 5

Skin Warts

Question 6

Viruses that cause cutaneous papilloma?

Answer 6

HPV-1-4

Question 7

How does cutaneous papilloma develop?

Answer 7

Usually in childhood, adolesence
Contact at site of trauma
Recurrence is common

Question 8

How does mucosal papilloma develop?

Answer 8

Benign Head/Neck Tumors

Anogenital Warts

Question 9

Types of Benign Head and Neck Tumors?

Answer 9

Single Oral Papillomas and Laryngeal Papillomas

Question 10

Laryngeal papillomas are associated with which viruses?

Answer 10

HPV-6 and HPV-11

Question 11

Why are laryngeal papillomas dangerous for children?

Answer 11

Can obstruct the airway

Question 12

Clinical Presentation of genital warts?

Answer 12

Squamous epithelium of external genitalia

Cauliflowerlike lesions

Question 13

Papilloma viruses associated with genital warts?

Answer 13

HPV-6 and HPV-11

Question 14

Papilloma viruses associated with cervical dysplasia?

Answer 14

16, 18, 31, 45

Question 15

HPV is strongly associated with…

Answer 15

Cervical cancer

Question 16

How does HPV cause cancer?

Answer 16

Breaking circular genome btw E1 and E2 – stops viral replication
E6 and E7 (oncogenic) are no longer regulated by E2
E6 and E7 inactivate P53 and RB

Question 17

Pathogenesis of HPV?

Answer 17

Invades basal cell layer
Latent Phase
Viral production begins in terminally differentiated cells
Leads to morphologic atypical koilocytosis

Question 18

What are koilocytes?

Answer 18

Multinucleated, Nuclear Enlargement, Nuclear hyperchromasia, perinuclear halo

Question 19

HPV acquired through….

Answer 19

Direct contact, sex, birth

Question 20

Control of Papilloma virus?

Answer 20

Podophyllin resin and Podofilox lotion – Remove Warts
Aldara cream – Immune Modifyer
Gardasil – 6, 11, 16, 18

Question 21

Types of Polyomaviridae?

Answer 21

SV40
Murine Polyoma
BK and JC Viruses

Question 22

Do BK and JC usually cause disease?

Answer 22

No. Only in immunocompromised.

Question 23

Site of JC and BK replication?

Answer 23

JC – Respiratory, Urinary, and CNS

BK – Respiratory and Urinary

Question 24

BK Virus Symptoms

Answer 24

Ureteral Stenosis (renal transplant recipients)
Hemorrhagic Cystitis (Bone Marrow Transplant recip.)

Question 25

Main JC Virus Symptom

Answer 25

Progressive Multifocal Leukoencephalopathy

Question 26

What is Progressive Multifocal Leukoencephalopathy?

Answer 26

Infects Oligodendrocytes, Loss of White Matter

Paralysis and Death

Question 27

Pathogenesis of JC and BK?

Answer 27

Enter respiratory, infect lymphocytes and kidney
Latent Infection of kidney (JC also B Cells+mono)
Viral Shedding in urine – UTI
Viremia and CNS infection (JC virus)

Question 28

Control of BK/JC?

Answer 28

None