other Dye and Stains Flashcards
Local Acting Dyes list:
- Fluorescein Dye
- Fluorexon
- Fluress
- Rose Bengal Dye
- Indocyanine green
- Argyrol
- Alcian Blue
- Sulforhodamine B
- Methylene Blue
- Trypan Blue
- Lissamine Green
Stains:
- Large Epithelial defects
- devitalized cells and mucin
- (stains cytoplasm & nucleus)
Fluorexon
Stains devitalized cells and mucin to brilliant RED
seen by naked eyes
Rose Bengal Dye
Stains skin of the eyelids
Rose Bengal dye
Stains nucleus more than the cytoplasm
Rose Bengal Dye
vital stain, stain devitalized cells & mucin
Acidic and used as food colorant
simple screening method for detection of vit. A deficiency (xerophthalmia)
LISSAMINE GREEN
protein bound given IV angiography vs. Fluorescein angiography
INDOCYANINE GREEN (ICG) angiography
Angiography – more effective in revealing choroidal neovascular membranes due to larger wavelengths
vs Fluorescein dye angiography
INDOCYANINE GREEN (ICG)
stains disease or dead corneal endothelial cells → intracameral injection
INDOCYANINE GREEN (ICG)
Stain anterior lens capsule in cataract surgeries (phacoemulsification) – using 0.5% intracameral injection
INDOCYANINE GREEN (ICG)
staining internal limiting membrane – using 0.05% to 0.5% for posterior segment surgeries → intravitreal injection
INDOCYANINE GREEN (ICG)
Corneal stromal dystrophy → characterized by deposition of acid muco-polysaccharides in the corneal stroma
stain by:
1% ALCIAN BLUE
Differentiate mucin deposits (stained blue) from diseased cells (unstained)
1% ALCIAN BLUE
- silver protein with antiseptic property
*1%-2% AgNO3 (Crede’s prophylaxis) → ophthalmia neonatorum infected Neisseria gonorrhea passes to infected birth canal
ARGYROL 10% - 20%
excellent indicator of adequacy of preoperative preparation prior to surgical eye scrub
(absence of stain means preparation is complete)
10% - 20% ARGYROL
molecular weight: 559
(does not stain epithelial cells but stain corneal stroma)
SULFORHODAMINE B
orange excited by green light
by viewing thru orange filter can eliminate fluorescence of sclera allowing observation of tear film
SULFORHODAMINE B
vital stain of corneal nerve tissue and stain devitalized corneal cells & mucin
5% METHYLENE BLUE
- bluish ocular discoloration persisting for 1 day
- has bacteriostatic property
5% METHYLENE BLUE
for irrigation of lacrimal sac prior to Dacryocystorhinostomy
5% METHYLENE BLUE
stains anterior lens capsule during phacoemulsification (w/o staining the lens cortex and nucleus)
– 0.06 % intracameral
TRYPAN BLUE
stain the ERM in posterior segment surgeries
(Epi-Retinal-Membrane)
TRYPAN BLUE
-indicator of corneal endothelial integrity for corneal grafting by injecting to A.C.(intracameral)
TRYPAN BLUE
stain and strip the Descemet’s membrane in Descemet’s stripping endothelial keratoplasty (DSEK)
TRYPAN BLUE
aid in stripping the corneal endothelium from the donor button in Deep anterior lamellar keratoplasty (DALK).
TRYPAN BLUE
stain the Tenon’s capsule after enucleation surgeries
TRYPAN BLUE
Dyes with Antimicrobial Properties:
Rose Bengal - anti viral
Lissamine Green - anti viral
Methylene Blue - anti bacterial
Argyrols - anti bacterial
used for Diagnosis of Dry Eye Syndrome (DES)
LISSAMINE GREEN
used In Sjogren’s syndrome suspects – to rule out Keratoconjunctivitis sicca
LISSAMINE GREEN
used for early diagnosis of KCS & Sjogren’s syndrome (tear deficiency)
ROSE BENGAL DYE
used for characterizing the dendritic lesions of Herpes simplex I & zoster lesions and superficial punctate keratitis
ROSE BENGAL DYE
diagnosis of Meibomian gland dysfunction (MGD) → oily layer Tear film def. → Dry Eyes Syndrome
ROSE BENGAL DYE
for diagnosis of polypoidal choroidal vasculopathy, choroidal neovascular membrane, age related macular degeneration (wet), and posterior uveitis
INDOCYANINE GREEN
Does not stain most soft contact lenses
FLUOREXON
prevent diffusing to contact lens
does not stain small epithelial defects e.g. micropunctate keratitis, fine dendritic keratitis, small blebs, minor corneal erosions
Fluorexon
