Fluorescein Dye

Question 1

ability of a molecule to absorb light of a lower wavelength and emit light of a higher wavelength

Answer 1

Fluorescence

Question 2

Fluorescein Dye is Orange-red in crystalline powder but _____ in solution.

Answer 2

yellow

Question 3

Fluorescein dye turns _____ with cobalt-blue light

Answer 3

Yellow-green

Question 4

Fluorescein strip dosage:

Answer 4

0.6 mg - 1 mg

Question 5

Fluorescein topical solution dosage:

Answer 5

0.1% - 0.2%

Question 6

Fluorescein Angiography FA solution preparations:

Answer 6

10% dye in a 5ml vial

20% dye in a 2ml vial

Question 7

what is the term called when the - Fluorescein pH – increase fluorescence at pH 8 but loses fluorescence at > pH: 8

or when

0.001-0.002mg – increase fluorescence and loses fluorescence at >0.001mg

Answer 7

Quenching Phenomenon

Question 8

when fluorescein dye dimerizes & polymerizes and emission shift to longer wavelength

Answer 8

Quenching phenomenon

Question 9

Clinical Characteristics of Fluorescein Dye:

Answer 9

1. Stains epithelial defects (MOA) bright green by diffusing into intercellular spaces
2. Will not stain devitalized/dead cells or mucus
3. Tear film appears yellow-orange
4. Promotes growth of Pseudomonas aeruginosa
5. Will stain soft contact lens → yellow tinge
6. Pseudo-flare
7. Fisher-Schweizer’s corneal mosaic or Anterior corneal mosaic

Question 10

Fluorescein dye stains ____ defects

Answer 10

epithelial defects

Question 11

Fluorescein dye Stains epithelial defects into ______ color by diffusing into intercellular spaces

Answer 11

bright green

Question 12

Fluorescein dye will not stain:

Answer 12

devitalized/dead cells

devitalized/dead mucus

Question 13

Fluorescein dye promotes growth of ________ bacteria

Answer 13

Pseudomonas aeruginosa

Question 14

in Fluorescein dye tear film appears ______ in color

Answer 14

yellow-orange

Question 15

Fluorescein dye will stain soft CL into _______

Answer 15

yellow tinge

Question 16

happens when - large epithelial breaks (corneal ulcer), fluorescein diffuses the corneal stroma and into anterior aqueous chamber producing greenish glow

Answer 16

Pseudo-flare

Question 17

fishnet-like mosaic pattern of fluorescein in tear film after rubbing the eye w/c disappear if it is wash out

Answer 17

Fisher-Schweizer’s corneal mosaic or Anterior corneal mosaic

Question 18

Indications for Fluorescein dye:

Answer 18

1. TBUT - evaluate tear film layer
2. Jones test - lacrimal drainage system
3. Aqueous wound leakage
4. RGP fitting pattern
5. Fluorescein Angiography - visualize Retina & choroidal blood flow
6. Goldman applanation Tonometry
7. Identify presence of Atypical Mycobacterium, fungi (Fusarium) & Acanthamoeba infection (Protozoa)
8. Study of KCS, DDT, keratitis association with diabetic retinopathy (fundus camera or OCT)

Question 19

– detect aqueous flow in conjunctival filtering blebs after trephine operation, corneal laceration or post-cataract operation

Answer 19

Seidel’s test

Question 20

in seidel’s test:

*Fluorescein is instilled on eye and dye is seen flowing downward from surgical or penetrating wound appearing as _______ in contrast to duller, yellow-orange background.

Answer 20

bright green

Question 21

pattern seen when eye is stained if Px has Herpes simplex I (H. keratoconjunctivitis) :

Answer 21

Dendritic Pattern

Question 22

test to demonstrate human lacrimal ductules after dacryocystorhinostomy

– reliability indices for anatomical and subjective success after dacryocystorhinostomy

Answer 22

Dye Disappearance Test

*Fluorescein dye disappearance test (DDT)

Question 23

Side effect of Fluorescein Dye:

Answer 23

eye irritation on cornea & conjunctiva