ML9

Question 1

Only species that matters for assignment

Answer 1

Arthopleura sola

Question 2

Concern

Answer 2

Green is not due to environment

Neon green animals found all over

Individual animals stay neon green

Question 3

Are the two images diff species

Answer 3

No

Found by DNA sequencing COXI gene from neon and WT animals

Question 4

Identifying the protein responsible

Answer 4

Take the total proteins

Discard all proteins except the ones that fluoresce green

Question 5

Edmon degradation sequencing

Answer 5

Take an amino acid chain and remove each one starting from the beginning of the chain

Question 6

Sanger DNA sequencing

Answer 6

Removing mRNAs from the neon

Turn them into complementary DNA

Sequence those DNAs

Question 7

What changes do they find between WT and neon

Answer 7

Single nucleotide polymorphisms (SNPs)

Can cause silent mutations

Found one missense mutation (Glycine at location 145 gets changed to ASP)

Question 8

Allele interaction

Answer 8

Incomplete dominance

Question 9

E coli experiment

Answer 9

Taking the gene and adding it to E coli to make a GFP

Added between the promoter and terminator

Question 10

Gene is added to

Answer 10

Start of protein