Lec 21

Question 1

Functions of cytoskeleton

Answer 1

Resist compression (done by Microtubules- pushed against the edge of the cell and tube shaped to resist compression)

Resist tension (done by actin and intermediate filaments)

Move chromosomes during mitosis (done by Microtubules)

Divide cell during cytokinesis (done by microtubules and actin)

Question 2

Toxin

Answer 2

Affects the cytoskeleton

Interferes with the breakdown or formation of protein filaments

Question 3

Taxol

Answer 3

Source- fungus living on pacific yew tree

Inhibits microtubules

Causes microtubules to grow and get stuck, leading to a big ball of microtubules being formed

Question 4

Taxol causes the cell to get stuck in

Answer 4

Mitosis because the microtubules cannot separate the chromosomes during anaphase

Question 5

Colchine

Answer 5

Source- autumn crocuses

Causes the microtubules to be unable to grow,

The microtubules get stuck when they shrink

Question 6

Colchine consequence

Answer 6

Cells get stuck in mitosis

Question 7

Phalloidin

Answer 7

Source- death cap mushrooms

Inhibits F-actin from turning into G-actin

Question 8

Phalloidin consequence

Answer 8

Tough to tell because the mushroom has worse ingredients

Question 9

Phalloidin mechanim

Answer 9

Phalloidin can be attached to actin which is then attached to a fluorescent dye so that it is visible

Question 10

Acrylamide

Answer 10

Synthetic used in PAGE (protein gel)

Breaks down IFs into keratins

Question 11

Acrylamide consequences

Answer 11

Kills sensory neurons