Lec 11 Flashcards
Vesicle
Phospholipid sphere with cargo proteins
How are vesicles formed
Cargo molecules stick to cargo receptors
Clathrin (and other coat protein) bind to flat membranes and bend it into balls, forming vesicles
Coat proteins
Surround vesicles
Clathrin
When put together they form soccer ball shapes
Adaptin
Connects coat proteins/clathrin to cargo receptors
Dynamin
Closes the vesicle and separates the vesicle
Removing coat proteins
Adaptin is regulated by Ran
It uncoats the protein by when the vesicle is made and Adaptin GTP turns into the inactive Adaptin GDP
Iron transport in humans
Red blood cells and hemoglobin
Iron delivery
Intestine cell
Blood
Future RBC
Transferrin proteins
Made in livir
Transports iron to target
Uses receptor mediated endocytosis
How to vesicles move
Microtubules
Microtubules have polarity and a characteristic arrangement in animal cells
Motor proteins when given ATP can walk along microtubules
Kinesin
Move from - end to + end along microtubules while holding onto vesicles
Dynein
Move from + end to - end along microtubules while holding onto vesicles
Receptor mediated endocytosis uses
Dynein
Golgi surrounds the
Centrosome
Microtubules come out of
Centrosome
Moving toward the centrosome is
Dynein
Moving away from the centrosome is
Kinesin
How do vesicles fuse with their targe membraines
SNAREs
v SNARE
Vesicle SNARE
in vesicle
t SNARE
Target SNARE
in plasma membrane
Vesicle leaving an organelle are given
The appropriate V-snare
Vesicles fuse with the target membrane when
SNAREs pull together
Later the cells are separated and
The v-SNARE goes back
