Lec 1-1 Flashcards
Light microscope includes
Light
Specimen
Eyepiece
Illumination of light microscope
UV or Visible light
Brightfield microscopy
Visible light passes through specimen
Problem with brightfield microscopy
We cannot see very much and stains are needed
Phase contrast microscopy
Visible light passes through specimen
Complex lenses makes darks darker and lights lighter
Differential Interference Contrast (DIC) microscopy
Visible light passes through specimen
Complex lenses makes darks darker and lights lighter
Puts a shadow on cells
Widefield fluorescence microscopy
Blue light is used on stained specimen (stained with fluorescein)
Green light is reflected
Filter only allows blue light into the system and only allow greenlight to pass through the eyepiece to be observed
Microscopy images involve
Either multiple images placed on top of each other or one image
This is done by individually searching for different colors and then superimposing all these images on top of each other
Where two images overlap their colors combine
Whenever using light use
Additive rules
Red + Green
Yellow
Purple + Green
White
Confocal fluorescence microscopy
Shows a 2D optical plane within a 3D specimen
Uses a laser to project light
More expensive
More detailed image
Tomography
Several confocal fluorescence can be assembled to make a 3D model
combining 2D images to make a 3D one
Getting specimen onto the slide
Pull up a cell culture and put a coverslip on top
Making things visible
Colored stains
Fluorescence stain
Fluorescent probes
