MicroBio 18: Microbial Biotechnology

Question 1

Any technique that uses living organisms/ substances from those organisms, to make or modify a product, improve plants or animals, or to develop microorganisms for specific uses (OTA, 1989)

Answer 1

Biotechnology

Question 2

the controlled and deliberate manipulation of biological systems (bio-whether cells or cell components) for the efficient manufacture or processing of useful products (technology)

Answer 2

Biotechnology

Question 3

Biotechnology products that uses microbes in genetic engineering

Answer 3

• GE plants with resistance to pests, diseases, and adverse environmentsl condition
• GE plants with increased yield and nutritional value
  _ GE plants with production of Antisera (antibody)
• Vaccines through Recombinant DNA technology
• Mass production of proteins (insuling and growth hormone) in bacterial cloning vectors for diabetes and dwarfism treatment
• Bioremediation
• Food and energy production
• Microbial Mining

Question 4

Transgenic corn with _______ gene which makes it resistant to ________

Answer 4

Bacillius thuringiensis; Asian corn borer

Question 5

Transgenic eggplant with Bacillus thuringiensis gene which makes it resistant to _______

Answer 5

fruit and shoot borer

Question 6

Transgenic cotton with Bacillus thuringiensis gene which makes it resistant to _______

Answer 6

Cotton bollworm

Question 7

Transgenic papaya that has the ________ of ________ makes it resistant to the virus

Answer 7

coat protein; papaya ringspot virus

Question 8

Transgenic zucchini with resistance to ______

Answer 8

zucchini-yellow mosaic virus

Question 9

________ is a genetically modified Rice with _______ genes that is a precursor to Vitamin A from _______ and a bacterium __________

Answer 9

Golden rice; beta carotene; daffodil; Erwinia uredovora

Question 10

Production of ______ or antibody for specific plant and animal pathogens that greatly facilitated their detection and disease diagnosis

e.g. antisera for cucumber mosaic virus ( CMV) detection

Answer 10

antisera

Question 11

what is CMV

Answer 11

Cucumber Mosaic Virus

Question 12

Vaccines are produced through ______

Answer 12

Recombinant DNA technology

Question 13

What is DPT vaccine

Answer 13

Diptheria, perstussis and tetanus

Question 14

Vaccine against polio

Answer 14

Polio vaccine

Question 15

Vaccine used for preventing COVID -19 caused by the SARS-CoV-2 virus

Answer 15

SARS Cov 2 vaccine26

Question 16

These proteins are mass produces in bacterial cloning vectors for the treatment of diabetes and dwarfism

Answer 16

Human insulin and growth hormone

Question 17

this inhibits cell proliferation and modulate the immune system

Answer 17

Human interferon

Question 18

this made DNA technology (genetic engineering) possible
e.g. EcoR1 enzyme - from E. coli cuts the DNA -equence AT|GAATTCG
TACTTAA|GC

Answer 18

Restriciton enzymes/ Restriction endonucleases/ molecular scissors

Question 19

use of microbes to degrade toxic wastes

Answer 19

Bioremediation

Question 20

Can microorganisms be used in food and energy production? and how?

Answer 20

Yes, by edible microbes, microbial fermentation and alcohol

Question 21

use of microbes to leach out mineral ores

Answer 21

Microbial mining