L06: Growth Nutrition And Identification Of Bacteria Flashcards
What is the difference between prokaryotes (bacteria) and eukaryotes (human cell)
Prokaryotes have dna free in cytoplasm
Eukaryotes have dna within a nucleus
What are bacteria classified into
Gram positive
Gram negative
What colour does gram positive stain
Purple
What colour does gram negative stain
Pink
What is the difference in structure of gram positive and negative
Gram positive: has one wall of peptidocyglan
Gram negative : has 2 walls of peptidocyglan and phospholipid membrane e
How are bacteria isolated in labs
From clinical samples
What does the clinical samples involve
Blood Stool Tissues Respiratory tract CSF
How are bacteria detected in the samples obtained
Matrix assisted laser desorption ionisation of time flight mass spectrometry (MLADI-TOF MS)
What does MALDI-TOF MS involve
Bacteria colony exposed to laser beam which ionises the sample, fragmentation products generated are identified using the database.
How do bacteria cause disease
1) attach to host
2) invade the tissue
3) acquire the nutrients to grow
4) Cause damage
How to bacteria attach to host cells
Use pili to teach to specific host receptors
How do bacteria invade the cell
Type 3 secretion system which promotes invasion into host cell
What main nutrient does bacteria acquire in the host cell to survive
Iron
How does the bacteria avoid the host immune system
Inhibit opsonisation
Inhibit phagocytosis
Inhibit antigen presentation etc.
What are the 2 mechanism damage to the host cell be made
Direct
Indirect
