Growth And Nutrition Of Bacteria Flashcards
Are bacteria eukaryotes or prokaryotes
Prokaryotes
Why are bacteria prokaryotes
The DNA is free in the cytoplasm
What are bacteria classed into
Gram positive
Gram negative
What are the structures of gram positive bacteria
Peptifoglycan
Lipoteichoic acid
Teichocic acid
What is the structure of gram negative cells
Peptidoglycan
Phospholipid
Lipopolysccharides
Which classification of bacteria has a double membrane
Gram negative
What colour does gram negative stain
Pink
What colour does gram positive stain
Purple
What is peptidoglycan a target of
Penicillin (antibiotics)
How can be collect bacteria in clinical samples
Blood Stool Urine Tissues Respiratory tract CSF
What does MALDI-TOF MS stand for
Matrix associated laser desorption ionization time of flight mass spectrometry
What is the purpose of a MALDI-TOF MS
To identify bacteria
How does a MALDI-TOF MS work
1) bacterial colony is exposed to a laser beam which ionises the sample
2) fragments are produced which is used to identify the organism by comparing with database
What are the 5 things bacteria has to do to cause disease
1) attach to host
2) invade the tissue
3) acquire nutrients and grow
4) avoid the host immune response
5) cause damage
How does bacteria attach to the host cell
Bacteria uses pili to attach to host cells
Attachment to specific receptors allows bacteria to be taken up and not killed
How do bacteria invade the cell
By type 3 secretion system to promote invasion into host cell
What nutrients do bacteria acquire
Iron
How do bacteria avoid the host immune response
Inhibit phagocytosis Inhibit antigen presentation Inhibit opsonisation Inhibit cytokine synthetics Inhibit complement Etc
What are the 2 mechanism bacteria can cause damage to the host cell
Direct mechanism
Indirect mechanism
What does the indirect mechanism of damage involve
Production of toxins and neurotoxins
What does the indirect mechanism of damage involve
Overactivation of inflammation
Molecular mimicry
What is molecular mimicry
Where microbial components of the bacteria are similar to the host
