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IIH > Growth And Nutrition Of Bacteria > Flashcards

Growth And Nutrition Of Bacteria Flashcards

1
Q

Are bacteria eukaryotes or prokaryotes

A

Prokaryotes

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2
Q

Why are bacteria prokaryotes

A

The DNA is free in the cytoplasm

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3
Q

What are bacteria classed into

A

Gram positive

Gram negative

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4
Q

What are the structures of gram positive bacteria

A

Peptifoglycan
Lipoteichoic acid
Teichocic acid

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5
Q

What is the structure of gram negative cells

A

Peptidoglycan
Phospholipid
Lipopolysccharides

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6
Q

Which classification of bacteria has a double membrane

A

Gram negative

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7
Q

What colour does gram negative stain

A

Pink

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8
Q

What colour does gram positive stain

A

Purple

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9
Q

What is peptidoglycan a target of

A

Penicillin (antibiotics)

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10
Q

How can be collect bacteria in clinical samples

A 
Blood 
Stool
Urine 
Tissues 
Respiratory tract
CSF
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11
Q

What does MALDI-TOF MS stand for

A

Matrix associated laser desorption ionization time of flight mass spectrometry

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12
Q

What is the purpose of a MALDI-TOF MS

A

To identify bacteria

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13
Q

How does a MALDI-TOF MS work

A

1) bacterial colony is exposed to a laser beam which ionises the sample
2) fragments are produced which is used to identify the organism by comparing with database

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14
Q

What are the 5 things bacteria has to do to cause disease

A

1) attach to host
2) invade the tissue
3) acquire nutrients and grow
4) avoid the host immune response
5) cause damage

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15
Q

How does bacteria attach to the host cell

A

Bacteria uses pili to attach to host cells

Attachment to specific receptors allows bacteria to be taken up and not killed

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16
Q

How do bacteria invade the cell

A

By type 3 secretion system to promote invasion into host cell

17
Q

What nutrients do bacteria acquire

A

Iron

18
Q

How do bacteria avoid the host immune response

A 
Inhibit phagocytosis 
Inhibit antigen presentation 
Inhibit opsonisation 
Inhibit cytokine synthetics 
Inhibit complement 
Etc
19
Q

What are the 2 mechanism bacteria can cause damage to the host cell

A

Direct mechanism

Indirect mechanism

20
Q

What does the indirect mechanism of damage involve

A

Production of toxins and neurotoxins

21
Q

What does the indirect mechanism of damage involve

A

Overactivation of inflammation

Molecular mimicry

22
Q

What is molecular mimicry

A

Where microbial components of the bacteria are similar to the host

IIH (26 decks)

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