JAALAS Sep 2016 random questions Flashcards

1
Q

A recent study indicates that embryo transfer in mice may have an effect on emotional behaviors of male or female offspring

A

Females more sensitive than males

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2
Q

T or F

A rhesus transgenic model of Huntington disease has been established

A

T.

The animals have just reached puberty and attempts at sperm collection and preservation are in process

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3
Q

What is the current optimal freezing material used for sperm preservation?

A

TEST extender (egg yolk + TES+ Tris buffer)

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4
Q

In a recent study TEST EQ was determined to be a better freezing extender for transgenic rhesus sperm.
What is TEST EQ?

A

Contains the detergent Equex paste, to increase acrosome and membrane integrity

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5
Q

What is the primary route of sensible water loss in mice?

A

Urine (sweat in minimal since they lack sweat glands, except in the foot pad)

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6
Q

What is the source of insensible water loss?

A

Respiratory route and skin, independent of physiologic state

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7
Q

How is TEWL (trans epidermal water loss) measured?

A

Using an evaporimeter (aka TEWL probe)

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8
Q

Are the SKH strain of mice haired or nude?

A

immunocompetent hairless mice

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9
Q

T or F

This recent study shows significantly higher TEWL in C57 compared to other strains studied

A

T

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10
Q

List some of the antioxidant enzymes in the body, used to combat ROS (Reactive oxygen species)

A

Superoxide dismutase, catalase, glutathione peroxide, glutathione reductase

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11
Q

Which anti oxidant enzyme is able to transform H2O2 into water?

A

Glutathione peroxide (GSH-Px)

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12
Q

Malondialdhyde is a break down product of what body tissue?

A

polyunsaturated lipids

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13
Q

What is the recommended time of recovery following transport for cynomolgus monkeys to recover from oxidative stress associated with transport?

A

21d

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14
Q

What is the most commonly isolated pinworm to naturally infect laboratory rodents?

A

Aspiculuris tetraptera

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15
Q

Aspicularis can elicit a Th1 or Th2 response from the host ?

A

Th2

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16
Q

Does Aspicularis have a direct or indirect life cycle?

A

direct

17
Q

What is the lifespan of adult Aspiculuris?

A

45-50d, reside in the proximal colon

produce eggs multiple time during the lifespan (unlike syphacia which produce only once)

18
Q

T or F

Aspicularis eggs are readily detectable on fecal floatation.

A

T

Adult worms shed eggs in the mucus covering the rodent feces

19
Q

What is the pre patent period of the Aspicularis eggs

A

21-25d

5-8d to embryonate

20
Q

Are male or female mice more susceptible to heavy pinworm burdens?

A

Male

21
Q

This recent articles suggests the following improvements to using fecal flotation for diagnosis of Aspiculuris:

A
  • soaking the eggs for 30min prior to mixing
  • 15min floatation period
  • collect at least 20 pellets per come or the entire overnight fecal output
  • time of day, sex, housing conditions did not have an effect
  • younger mice often yield better results on fecal flotation
22
Q

This recent study determined that intraperitoneal CRI of KXA was successful at providing prolonged continuous surgical anesthesia and immobility in mice.

A

isoflurane is still more effective, but when this is not available IP CRI may be more useful than bolus repeated dosing

23
Q

Is ketamine an NDMA agonist or antagonist?

A

antagonist

binds to the phencyclidine receptor and blocks the NMDA receptor channel

24
Q

Name the hepatic microsomal enzymes that metabolize ketamine

A

CYP3A4

25
Q

This recent study indicates an effect of ketamine on energy and amino acid metabolism in cynomolgus macaques

A

specifically metabolites related to glycolysis and glyconeogenesis pathways
-Serum levels of lactate, a-glucose and myoinositol and urine levels of lactate pyruvate and succinate were decreased

26
Q

What is the biologic half life of ketamine?

A

2.5-3hour

27
Q

Genus species of the rainbow trout

A

Oncorhynchus my kiss

28
Q

What is the mechanism of action of Meditomidate in fish?

A

binds to GABAa receptor, enhances its function causing hypnotic and immobilized state

29
Q

What is the mechanism of action to Tricaine Methanseulfonate?

A

binds to sodium channels and reduces depolarizing current

30
Q

A recent study determined that exposure of rainbow trout to TMS or Metidomidate prior to euthanasia did not influence contractility of myocardium

A

T

31
Q

What does the submental region refer to with regard to blood collection from mice?

A

the vasculature under the chin

32
Q

Genus species of the land snail

A

Succinea putrid

33
Q

From the recent JAALAS article what is an acceptable method to euthanize terrestrial invertebrates

A

Anesthetize first with low percentage alcohol (beer or 5%) for 10min, then euthanize in 70-95% etoh or 10%formalin

34
Q

What is the mechanism of action of Alphaloxine

A

Bind to GABAa cell surface receptors

may cause hypotension