JAALAS Nov 2016 random questions

Question 1

T or F
Brief isoflurane anesthesia for oral gavage dosing in mice showed no evidence of greater stress than oral dosing awake mice

Answer 1

T

Question 2

What has been the acceptable standard of RLUs in santiziationn of laboratory animal settings

Answer 2

1000RLUs

Question 3

T or F

EAD sampling using PCR is sensitive even when disease prevalence is low

Answer 3

T

Question 4

How often should enclosures and accessories (tops) be sanitized according to the guide?

Answer 4

at least once every 2 weeks

Question 5

The dose of Dexmeditomidine required to achieve the equivalent effect of Meditomidine is 1/2 as much or 2x as much?

Answer 5

1/2 the dose of Dexmeditomidine is required

Question 6

What does the term “piping” refer to in zebra fish

Answer 6

gasping for air at the water surface

Question 7

T or F.
A recent study in JAALAS 2016 determined that neither bacterial growth or ATP concentration differed significantly between 1 and 4 weeks of use (in mice housing)

Answer 7

T

Question 8

This recent study shows that BALB/c mice euthanized by slow filled CO2 chamber develop severe pulmonary hemorrhage

Answer 8

This may need to be refined in a balb/c study with pulmonary pathology as an endpoint

Question 9

genus sp of Sooty Mangaby

Answer 9

Cercocebus atys

Question 10

T or F European rabbits are know to live in social groups with dominate male and several subordinate males and females

Answer 10

T

this is unique to european rabbits

Question 11

T or F

Lidocaine has the same mechanism of action as MS222

Answer 11

T

Question 12

Traditional cryopreservation medias contain egg yolks extenders to aid with preservation. What is the risk of using hen egg yolk for preservation of sperm

Answer 12

potential pathogens- viruses (influenza), fungus

Question 13

T or F.
Administration of Ketamine xylazine cocktail by SC route can be effective for surgical anesthesia in some strains and sexes of mice

Answer 13

T

this article determined it to be safe and effective, but may need some refinement of appropriate dose per strain and sex

Question 14

Ketamine inhibits/activates N-methyl-D-aspartate receptors

Answer 14

inhibits

Question 15

What is the most reliable and sensitive test method to detect Pasteruella pneumotropica?

Answer 15

PCR

serology unreliable, mass spect too expensive

Question 16

T or F. Acarus siro are visible to the naked eye

Answer 16

F.

The grain mite is generally not visible to the naked eye (o.5mm) compared to the book lice (3mm)

Question 17

What units are used to express the amount of ATP measured by the luminometer.

Answer 17

Relative light units (RLU)

Question 18

T or f
IVC exhaust sampling reliably detected pathogens that are difficult to detect by traditional sentinel housing, and detected pathogens weeks after the onset of pathogen detection

Answer 18

T

Question 19

Is xylazine a alpha-2 agonist or antagonist?

Answer 19

agonist

Question 20

What component in egg yolk is thought to aid with cryopreservation of sperm

Answer 20

LDL

Question 21

Buprenorphine is 25-50 times more/less potent than morphine

Answer 21

more

Question 22

Buprenorphine is a partial agonist at the ____ receptor and antagonist at the ___ receptor

Answer 22

mu

kappa

Question 23

T or F

C57BL/6 express Klra genes?

Answer 23

F

Klra genes are part of the NK cell that help with antigen presentation, 129 and C3H strains do express them

Question 24

genus species of the commonly use european rabbit

Answer 24

Orytolagus cuniculus

Question 25

Glucocorticoid levels in the hair is an indicator of chronic or acute stress?

Answer 25

chronic

Question 26

T or F. Mouse norovirus is persistently shed in the feces

Answer 26

T | as opposed to MHV ad MPV which are shed transient

Question 27

C57BL/6 predominately develop a Th1 or Th2 response | Balb/C predominately develop a Th1 or Th2 response

Answer 27

C57- Th1 | BALBc- Th2

Question 28

What is the product produced by flour beetles (Tribolium sp ) that renders and unpleasant oder and may induce tumor incidences in swiss albino mice

Answer 28

Benzoquinone

Question 29

T or F. Wild rabbits tend to socialize in groups of 4-6

Answer 29

F | They tend to socialize in groups of 3 or less

Question 30

T or F. Rabbits are a crepuscular species

Answer 30

T

Question 31

T or F. Sooty Mangaby troops are typically multimale-multifemale with a matrilineal dominance structure

Answer 31

T | Though less nepotistic than macaques or baboons

Question 32

Submissive acts between rabbits often include retreating when a dominant buck approaches. A safety distance of ___m seems to be the the observed minimal safety distance to demonstrate submission

Answer 32

1m

Question 33

Current guidelines recommend CO2 euthanasia using what percentage air displacement rate per minute

Answer 33

10-30% | And do not recommend placing conscious animals in a pre-filled chamber

Question 34

What is the most common chemical agent for euthanizing zebrafish. How?

Answer 34

MS222 | Dipolarizes sodium channels rendering unconsciousness, vascular collapse and death due to hypoxia

Question 35

T or F. | Sanitization consists of cleaning and disinfection

Answer 35

T | cleaning removes organic debris and disinfection then reduces the concentration of microbes

Question 36

Name a dissociative anesthetic that produces catalepsy .

Answer 36

ketamine

Question 37

T or F. | Dexmeditomidine is the dextroisomer of medetomidine

Answer 37

T

Question 38

What is the accepted date after birth when rats are considered adult?

Answer 38

60d

Question 39

What does the Randal-Selitto test measure?

Answer 39

Withdrawal response to pressure applied by an algeisometer | http://www.iitcinc.com/images/Randall-Seilto_new.jpg

Question 40

What does EAD stand for in relation the environmental sampling of IVC units

Answer 40

Exhaust air duct sampling

Question 41

Does Dexmeditomidine reduce or increases cerebral blood flow?

Answer 41

Reduces it | Through alpha-2 receptor mediated vasoconstriction

Question 42

T or F | Neither lidocaine nor MS222 are effective at euthanizing zebrafish less than 16dpf

Answer 42

T | According to this recent study

Question 43

Free ranging rabbits maintain an interindividual distance of how may meters during daytime actives

Answer 43

15-20m | suggesting even social groups require a lot of space

Question 44

Polyplax spinulosa is the vector for what common laboratory animal pathogen

Answer 44

Mycoplasma haemomuris

Question 45

A multientranced burrow system in which rabbits dwell is called a ______

Answer 45

warren

Question 46

Genus sp of booklice Genus sp of grain Mites Genus sp of Flour Beetles

Answer 46

Liposcelis spp Acarus siro Tribolium sp.

Question 47

T or F | larval stage zebra fish use gills more than skin for respiration

Answer 47

F. They use skin more than gills, which effects how chemical euthanasia agents (lidocaine and MS222) work in larva vs adults

Question 48

T or F. A recent study indicates that lidocaine hydrochloride was effective for euthanasia of adult zebrafish

Answer 48

T | And produced it more rapidly than did MS222

Question 49

Flour beetles can be an intermediate host for what laboratory animal pathogen known to infect rats, mice, humans and other primates, albeit rare.

Answer 49

Hymenolepis dimunata

Question 50

T or F. The size of a group and warren is related to the amount of "cover" present, since that is the rabbits main defense against predators

Answer 50

T. If cover is plenty, rabbits may diverse themselves, and breeding females may not share burrows and instead isolate themselves