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ACLAM > BMBL > Flashcards

BMBL Flashcards

1
Q

Which 2 organizations have created the risk group classification of agents?

A

WHO and NIH

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2
Q

What risk group is classified as “high individual risk and low community risk”

A

Agents in risk group 3
a pathogen that may cause serious human or animal disease, but does not easily spread, and for which effective treatment or prevention is available

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3
Q

What type of agents are found in risk group 4

A

cause serious or lethal disease, no treatment or prevention available

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4
Q

Coxiella burnetii is known to require a very high or very low infective dose?

A

very low, only a few infectious particle (10) are required for infection. It is also resistant to environmental stresses

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5
Q

Importation of etiologic agents of human disease are required from what government entity?

A 
CDC permit
(USDA permit required for importation of etiologic agents of animal diseases)
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6
Q

The NIH Guidelines for Research Involving Recombinant DNA Molecules is mandatory for whom?

A

Investigators conducting recombinant DNA research funded by NIH

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7
Q

What is the OBA

A

Office of Biotechnology Activities

responsible for oversight of recombinant DNA research

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8
Q

What is the RAC

A

The NIH recombinant DNA Advisory Board

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9
Q

What was the first document to formulate the idea of an institutional biosafety committee?

A

The NIH Guidelines for Research Involving Recombinant DNA

an IBC is required for all non-exempt experiments as defined by the NIH guidelines

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10
Q

What is the definition of recombinant DNA according to the NIH principles

A

the joining of natural or synthetic DNA segments to DNA molecules that can replicate in a living cell

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11
Q

Who is responsible for ensuring that personnel are aware of specific hazards and trained and proficient in microbiological procedures and techniques

A

The director or person in charge of the laboratory (the principle investigator)

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12
Q

How many classes of biosafety cabinets are there?

A

3

I, II, III

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13
Q

Which type of BSC provides the highest attainable level of protection to personnel and the environment

A

BSC Class III

gas-tight

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14
Q

Which BSC class does not provide protection from external contamination of the materials inside the cabinet

A

BSC I
-open front, designed to protect personnel and environment from material used under the hood, but does not protect the product (material being used under the hood)

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15
Q

Who is specifically and primarily responsible for assessing the risks and applying the appropriate BSL

A

The laboratory director

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16
Q

What is the primary facility requirement for BSL-1

A

a hand washing sink

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17
Q

T or F

BSL-2 practices require the use of a BSC

A

F

with good practices these agents can be used safely in activities conducted on the open bench

18
Q

T or F

Work with HIV can be performed at BSL-2 level

A

T

assuming the risk for producing splashes or aerosols is low

19
Q

Work with primary human derived blood, cells, fluid tissues, where the presence of an infectious agent may be unknown, should be done under BSL-2 or BSL-3 conditions

A

BSL-2

20
Q

T or F

BSL-3 standards require the use of a BSC or higher level of protection

A

T

21
Q

What are the primary hazard to personal working with agents in BSL-2?

A

accidental percutaneous inoculation of the agent

ingestion of agent

22
Q

What is the common BSL requirement of clinical/diagnostic specimens of unknown pathogen status?

A

BSL-2, unless there is a high suspicion of sever pathogen like hemorrhagic fever

23
Q

T or F

People must wash their hands after working with potentially hazardous agents in BSL-1 conditions

A

T

BSL-1 Laboratory must have a sink

24
Q

T or F

Mouth pipetting is only permitted when working in BSL-1 and not other BSL.

A

F

mouth pipetting is not permitted under any BSL conditions

25
Q

What should be on the sign to the door of a BSL-1

A

universal biohazard symbol

may also include: the name of agent, name and phone number of lab supervisor

26
Q

T or F

A pest control program is required for BSL-1

A

T

27
Q

T or F

Work in BSL-2 labs must use a Biosafety cabinet

A

F

only needed if work is prone to generate aerosols or splashes, or dependent on risk assessment

28
Q

What information must be included on the sign to the door of BSL-2

A
  • biohazard symbol
  • biosafety level of the lab
  • supervisor name
  • telephone number
  • procedures for entering and exiting
  • agent info in accordance with institutional policy
29
Q

T or F

A biosafety manual must be present in BSL-2 labs

A

T

must be available and accessible and adopted as policy

30
Q

T or F
Laboratory personnel must be provided with appropriate medical surveillance and offered immunizations, when working at BSL-2

A

T

31
Q

T or F

It is ok to have potted plants in a BSL-2 lab

A

F

no plants or animals unrelated to the study should be in the lab

32
Q

T or F

In BSL-2 labs, protective gear can be taken home for laundering?

A

F
Protective gear should be removed before exiting the lab, it can be disposed of or deposited in location for laundering (by the institution)

33
Q

T or F

BSL-2 laboratory doors should be self closing and have locks

A

T

34
Q

What is the ventilation requirement for BSL-2

A

No specific requirement

recommend inward flow with no recirculation to areas outside the lab

35
Q

HEPA filter exhaust air may be recirculated from a class I or class II BSC?

A

class II provided it is certified to be working correctly at least annually

36
Q

T or F

BSL-3 procedures only need to be conducted in a BSC if they are may cause aersolization

A

F

All BSL-3 procedures should be performed under a BSC

37
Q

T or F

The hand washing sink in BSL-3 laboratory can work by manual operation

A

F
must be hands-free or automatic
(differs from BSL-2 which can be manual or automatic/hands-free)

38
Q

T or F

All windows in a BSL-3 lab should have a screen

A

F
there should be no windows or the windows should be sealed
(this is different from BSL1 and BSL-2 which can have windows with screens)

39
Q

T or F

Volatile chemical hazards typically pass through HEPA filters?

A

F
Most chemical hazards will pass through HEPA filter, so 100% exhaust is required to work with these hazardd (BSC Class II type B2). In fact chemical hazards may degrade HEPA filters so the quanity of chemicals used may be limited

40
Q

T or F

Most animal facilities are designed with features and practices to meet BSL-1

A

T

unless the use of higher level pathogens requires higher a BSL

ACLAM (32 decks)

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