Introduction to Virology, Virus Striucture and Classification Flashcards
Study of Virus
Virology
HIstorical Background
When and Where was the 1st written record of a virus infection
- 1400 BC
- Memphis, ancient Egypt’s
Historical Background
How was the 1st record of virus was written or recorded?
through a Hieroglyph
Historical Background
who was depicted in the 1st written record?
Depicting Siptah showing typical clinical signs of paralytic POLIOMYELITIS
Siptah - temple priest
Historical Background
First report of viral infection (identified because of hieroglyph)
Poliomyelitis
what system is attacked by the Poliomyelitis?
CNS
Historical Background
Pharoah Ramses V is believed to have died/ succumbed to smallpox
1196 BC
Historical Background - 1996 BC
Pharoah Ramses V is believed to have died/ succumbed to?
smallpox
Historical Background
who pharoah have died because of smallpox in 1196 BC?
Pharoah Ramses V
Historical Background
how was the smallpox was identified to be seen during 1196 BC?
hirap mag english sensha
COMPARISON of the pustular lesion on the face of the mummy to those of more recent patient of smallpox
Historical Background
what virus family smallpox came from?
POX VIRUS (Eradicated, but WHO has a copy)
Historical Background - 1000 BC
Smallpox was endemic in China, in response, the practice of ____________ was developed
Variolation – A form of vaccination
Historical Background
what and when was the 1st attempt to vaccination?
- Variolation
- 1000 BC
Historical Background
how was variolation was made?
Crusted and powderized and was inhaled
Historical Background - 1000BC
TOF
BUT this was not completed proven that it came from smallpox virus (lack of documentation)
T
Historical Background
when was the vaccination James Phipps (8-yr old) using cowpox infected material of Sarah Nemes (milkmaid)
May 14, 1796
Historical Background
who received the vaccination during May 14, 1796 and where did the infected material came from?
- James Phipps (8-yr old)
- Sarah Nemes
Historical Background
the vaccination experiment on may 14, 1796 was headed by?
Edward Jenner
it was through injecting, different from variolation
Historical Background
when was Edward Jenner challenged the boy by deliberately inoculating him with material from a real case of small pox
July 1, 1796
Historical Background - association
what materials were used?
May 14 , 1796 :
July 1, 1798 :
May 14 , 1796 :: infected material from a milkmaid (Sarah Nemes)
July 1, 1796 :: Inoculated virus from a real case of small pox
General Characteristic of Virus
TOF
Obligate Extracellular Parasites
F (Intracellular)
Genetic elements that can replicate only inside a living cell
General Characteristic of Virus
TOF
Possess their own genomes
T
General Characteristic of Virus
TOF
grow in artificial culture media (In vitro)
F (can be in vivo by using cell culture, chick embryo, animlas; live animals)
General Characteristic of Virus
TOF
Largest infectious units
F (Small)
20 –300 nm in diameter
General Characteristic of Virus
TOF
Brightfield microscopy is required
F (Electron)
General Characteristic of Virus
Most Imporatant characteristic; They cannot exist, survive, and proliferate outside a living host cell
Being Obligate Intracellular Parasites
General Characteristic of Virus
DNA
Smallest and largest animal Virus
Parvovirus and Poxvirus
Viral Components
Viral Components of VIRUS
- Nucleic Acid Genome
- Capsid (protein coat)
Capsid
Capsid + genome =
Nucelocapsid (Virion)
Viral Components
made up of protein that protect viral genome
Capsid
Viral Components
covers the nucleic acid genome and protects it against adverse condition
Capsomeres
Viral Components
Repeating structural subunits used in capsid?
Capsomeres
Viral Components
what is used for envelope from the host cell membrane as a viral component?
Glycoprotein Spike
Capsid
who have and does not contain an envelope?
Naked Capsid (no envelope)
Enveloped Capsid (Has envelope)
Naked Capsid
main component?
Protein
Naked capsid - Properties
Environmentally STABLE to?
- Temperature
- Acid
- Proteases
- Detergents
- Drying
Released from cell by lysis
Naked capsid - Properties
TOF
Cannot dry out and will not retain infectivity
F (it can be dry but still infective)
Naked capsid - Properties
TOF
Can be spread easily
T (on fomites, from hand to hand, by dust, by small droplets)
Naked capsid - Properties
TOF
Can survive the adverse conditions of the gut
T
Naked capsid - Properties
TOF
Can be resistant to detergents and poor sewage treatmen
T
Naked capsid - Properties
TOF
Antibody may be not sufficient for immunoprotection
F
Enveloped Capsid
components?
- Membrane Lipids
- Proteins
- Glycoproteins
Enveloped Capsid
TOF
Environmentally LABILE-disrupted
T
Enveloped Capsid
Cannot be digested by Acid, Detergents, Drying, Heat
F (Can be)
Enveloped Capsid - properties
TOF
modifies cell membrane during replication
T
Enveloped Capsid - properties
TOF
Release by badding and cell lysis
F (Budding)
Enveloped Capsid - properties
TOF
Must stay WET to retain infectivity
T
Enveloped Capsid - properties
how is the enveloped capsid spread?
- Large Droplets
- Sectretion
- Organ Transplant
- Blood transfusion
Enveloped Capsid - properties
TOF
can survive the harsh environment of GI Tract
F
Enveloped Capsid - properties
TOF
Need to kill the cell to spread
F (does not need)
Enveloped Capsid - properties
TOF
May need antibody AND cell-mediated immune response for protection and control
T
Enveloped Capsid - properties
Elicits ____________ and ____________ to cause immunopathogenesis
- Hypersensitivity
- Inflammation
Viral Symmetry / Shape
what are the nucleoplasid structure?
- Icosahedral structure (cubic)
- Helical structure (also appears as spiral)
- Complex structure (seen in Poxviridae)
Viral Symmetry / Shape
onsisting of identical subunits that make up equilateral triangles that are in turn arranged in a symmetrical fashion
Icosahedral
Viral Symmetry / Shape
Protomers not grouped in capsomeres but bound to form a ribbon-like structure
Helical
Viral Symmetry / Shape
nucleic acid surrounded by a hollow protein cylinder or capsid and possessing a helical structure
Helical
Viral Symmetry / Shape
Viruses that do not fit the first two groups
Complex structure
Viral Symmetry / Shape
Exhibited by poxvirus and rhabdovirus
Complex Structure
Enumerate Viral Multiplication Cycle
kabisaduhin - VERY DEMURE
- Adsorption
- Penetration
- Uncoating
- Synthetic Phase
- Viral Assembly
- Release
Viral Multiplication Cycle
The viruses are very specific therefore, they have to recognize first the host cell with which they have a receptor for and then, it will undergo attachment with it.
Adsorption
recognition and attachment
Viral Multiplication Cycle
what is the important component for cell adhesion?
Cell Adhesion Molecules
Viral Multiplication Cycle - Adsorption
Taken advantage by virus has glycoprotein
glycoprotein - capsulated
Cell Adhesion Molecules
Viral Multiplication Cycle - Adsorption
Enumerate the Attachment Exemption Examples:
- Poliovirus - Immunoglobulin
- HIV - CD4+ Cells
- Influenza - Sialic acid (epithelial cell surface-exposed sialic acid receptors )
- Covid-19 - Angiotensin-converting enzyme 2 (ACE2)
- Epstein-Barr virus - C3D
Viral Multiplication Cycle
After it has attached, it should try to enter the host cell
Penetration
Viral Multiplication Cycle - Penetration
Naked ::
Enveloped ::
Naked :: Direct Penetration
Envelopped :: Usually Endocystosis or Cytoplasmic Vacouole
Viral Multiplication Cycle
When it has already penetrated the host cell, it will undergo?
Uncoating
Viral Multiplication Cycle
removal of nuceleoplasid to remove the lipid envelope by lysis or dissociation
Uncoating
Viral Multiplication Cycle
It will take over the nuclei of the host cell and it will dictate the metabolic processes of the cell.
Synthetic Phase
Viral Multiplication Cycle - Synthetic Phase
DNA ::
RNA ::
DNA :: Multiplies or assemble in nucleus
RNA :: Cytoplasm
Viral Multiplication Cycle
in synthetic phase the genome is produced in?
Nucleus
Viral Multiplication Cycle
in synthetic phase the viral proteins are produced in the
cytoplasm
Viral Multiplication Cycle
The protein component will assemble with the gene component.
Viral Assembly
Viral Multiplication Cycle
in releasing the virus, how it is for naked and enveloped?
Naked - lysis ; Enveloped - budding (ikaw) & lysis
Virus categories:
- 6 Orders (name ending in -virales)
- 87 Families (-viridae)
- 19 Subfamilies (-virinae)
- 348 Genera (-virus)
- 2290 Specie
who came up with the viral classification?
ICVT (International Committee on Viral Taxonomy)
4 Major Properties of Vriuses
- Types of Nucleic Acid
- Symmetry and Shape of the Capid
- Presence or Absence of Envelope
- Size of the Virus Particle
DNA Viruses
Clue: HHHAPPPY
- Herpesviridae
- Hepadnaviridae
- Hepatoviridae
- Adenoviridae
- Parvoviridae
- Poxviridae
- PaPoVaviridae
Rule: DNA viruses
what are the rules?
- All are DS DNA
- All Multiply in nucleus
- All are icosahedral
- All are enveloped
Rule: DNA viruses
All are DS DNA except
PARVOVIRIDAE
Rule: DNA viruses
All multiply in nucleus except
POXVIRIDAE
Rule: DNA viruses
All are icosahedral except
POXVIRIDAE (complex)
Rule: DNA viruses
All are enveloped except
PAP (PaPoVaviridae, Adenoviridae, Parvoviridae
RNA VIRUS
POSITIVE SENSE
Clue: Call Pico and Flavi To Come Right
- Calciviridae
- Picornaviridae
- Flavaviridae
- Togaviridae
- Coronaviridae
- Retroviridae
RNA VIRUS
what are the shape of positive sense
Icosahedral
RNA VIRUS
Negative Sense
Clue: Pairing Of File Rats at Bunny’s Area
- Paramyxoviridae
- Orthomyxoviridae
- Filoviridae
- Rhaboviridae
- Bunyaviridae
- Arenaviridae
RNA VIRUS
what are the shape of a negative sense?
Helical
What are the rules for RNA viruses
- All are SS RNA
- All are enveloped
- All are non-segmented
- Generall helical
Rule: RNA viruses
All are SS RNA except
REOVIRIDAE
Rule: RNA viruses
All are enveloped except
PCR (Picornaviridae, Calciviridae, Reoviridae
Rule: RNA viruses
All are non-segmented except
ROBA (Reoviridea, Orthomyxoviridae, Bunyaviridae, Arenaviridae)
RNA viruses
generally helical except?
positive sense RNA viruses
Biggest and Smallest RNA
- Paramyxoviridae (biggest)
- Enteroviridae (smallest)
if u see this card
kindlyt go to trnases and check why negative sense need to be converted to positive sense