ImmunoSero Lec (Midterms - Serologic Techniques)

Question 1

Spx used

Answer 1

Serum:

> Fresh
Non hemolyzed
Plain tube/red top
No RBC contaminant

Question 2

Complement inactivation

Answer 2

Heated to 56C for 30 minutes (When more than 4 hours has elapsed since inactivation, a specimen can be reinactivated by heating it to 56° C for 10 minutes)

Question 3

Spx. storage

Answer 3

2-8C (72 hours)
Frozen at -20C or below

Question 4

Marked and calibrated to deliver only 1 volume of specified fluid; Labeled as “TD” or “to deliver” with an oval bulb at het center and tapered at the dispensing end.

Answer 4

Volumetric pipette

Question 5

Suctioning device used by volumetric pipettes

Answer 5

Rubber bulb

Question 6

Volumetric pipettes should be held in this position with the tip against the surface of a container.

Answer 6

vertically

Question 7

Have markings that allow for varying amounts of liquids to be measured

Answer 7

graduated pipette

Question 8

Recognized by a frosted ring at the noncalibrated end, with calibrations extending to the tip; blow out pipettes; labeled as “TC”

Answer 8

serological pipette

Question 9

pipettes used when great accuracy is not required

Answer 9

graduated pipettes

Question 10

Allow rapid repetitive measurements and delivery
of predetermined volumes of reagents; Piston-operated; more accurate

Answer 10

Micropipette

Question 11

an indication of relative concentration

Answer 11

dilution

Question 12

How many tubes does a complete dilution series contain?

Answer 12

5 or 10

Question 13

If serum is being tested for antibody levels with a specific infectious organism, generally the blood should be drawn during the

Answer 13

acute phase of the illness

Question 14

when the disease is first discovered or suspected— and another sample drawn during the

Answer 14

convalascent phase (usually about 2 weeks later)

Question 15

reciprocal of the highest dilution of the patient’s serum in which the antibody is still detectable

Answer 15

Antibody titer

Question 16

indicates that a considerable amount of antibody is present in the serum

Answer 16

high titer

Question 17

For most pathogenic infections, an increase
in the patient’s titer of two doubling dilutions, or from a positive result of 1:8 to a positive result of 1:32 over several weeks, is an indication of a current infection. This is known as

Answer 17

fourfold rise in the antibody titer