BB LAB RED CELL SUSPENSION Flashcards
Liquid portion of the blood with anticoagulant
Plasma
Liquid portion of blood without anticoagulant
Serum
What tube is used in Red cell suspension to acquire serum?
Plain tube / Red top tube
Red cell blood suspension are prepared to wash off?
Excess protein and fibrin clots
Why do we need to wash off excess protein and fibrin clots?
To eliminate false reactions:
false positive and false negative
Materials used in Red cell suspension
Blood sample
Test tubes
Plain tube
EDTA tube
Centrifuge
Buffered normal saline
Final result of Red cell suspension
Bright Red
Composition of Normal Saline Solution
0.85% sodium chloride
How many drops of whole blood is transferred into the test tube?
2-3 drops
How many times should we wash red cell
3 times
Wash red cell three times with _____
Buffered normal saline
After the 3rd washing, how many drop/s of blood will be transferred to a test tube, and how many drops of buffered normal saline should be added?
1 drop blood
19 drops buffered normal saline
Centrifuge the blood for
3 mins
Normal Saline Solution should be within __________ concentration
0.85% to 0.90%