BB LAB (QC& Forward Typing) MIDTERMS Flashcards
Before performing your daily QC testing, inspect______
Inspect all reagents under test for evidence of contamination or deterioration
What are the things you need to record on the corQC Data Sheet
Lot number
Expiration date of each reagent
Observations
Tubes can be labeled as:
Anti-A
Anti-B
Anti-A,B
Anti-D
A1cells
B cells
How many drop/s of each reagent to the appropriate tubes?
1 drop of each reagent
How many drop/s of corQC reagent cells to the 1st four tubes?
1 drop of corQC reagent cells
How many drop/s of corQC Antiserum to the last 2 tubes?
1 drop of corQC Antiserum
Centrifuge all tubes for
30 seconds
Record results on the______
corQC reagent sheet
Principle of ABO forward typing
Testing with both Anti-A and Anti-B is necessary to determine if red blood cells possess or lack A and/or B blood group antigens
Absence of agglutination is a negative test result, which indicates______
the corresponding antigen is not demonstrable
Agglutination of red blood cells with a given reagent is a positive test result, which indicates______
the presence of the corresponding antigen on the red blood cells
To recognize reagent deterioration, the reagent must be_____
The reagent must be tested daily with appropriate controls
Reagents contain _____as preservative
Sodium azide 0.1%
Reagent should not be use beyond expiration date. T or F.
True
Storage of the reagent
Store at 2 to 8C
May be at room temp (20 to 30C) while in use
Specimen use in ABO forward typing tube method
EDTA anticoagulated blood
In ABO forward typing, sample should be stored at_____
2 to 8 C
No special preparation of the patient is required prior to specimen collection in ABO forward typing. T or F
True
How many drops of buffered NSS in making 3-5% red cell suspension
19 drops
How many drop/s of anti-A to tube “A”
1 drop
How many drop/s of anti-B to tube “B”
1 drop
How many drop/s of red cell suspension to the tubes “A” and “B”
1 drop
_____________ of red blood cells in the presence of reagent is a positive test result and indicates the presence of the corresponding antigen.
Agglutination
Negative control should have a ______
Negative result
State the common sources of technical error in ABO typing:
- Inadequate identification of blood specimens, test tubes or slides
- Cell suspension either too heavy or too light
- Clerical errors
- A mix-up in samples
- Missed observation of hemolysis
- Failure to add reagents
- Failure to follow manufacturer’s instructions
- Uncalibrated centrifuge
- Contaminated reagents/ expired reagents
- Warming during centrifugation due to uncalibrated centrifuge
One solid agglutinate (clear background)
+4
Several large agglutinates (clear background)
+3
Medium sized agglutinates
+2
Small agglutinates (turbid background)
Tiny agglutinates (turbid background)
+1 to W+
No agglutination or hemolysis
0
