BB LAB (QC& Forward Typing) MIDTERMS

Question 1

Before performing your daily QC testing, inspect______

Answer 1

Inspect all reagents under test for evidence of contamination or deterioration

Question 2

What are the things you need to record on the corQC Data Sheet

Answer 2

Lot number
Expiration date of each reagent
Observations

Question 3

Tubes can be labeled as:

Answer 3

Anti-A
Anti-B
Anti-A,B
Anti-D
A1cells
B cells

Question 4

How many drop/s of each reagent to the appropriate tubes?

Answer 4

1 drop of each reagent

Question 5

How many drop/s of corQC reagent cells to the 1st four tubes?

Answer 5

1 drop of corQC reagent cells

Question 6

How many drop/s of corQC Antiserum to the last 2 tubes?

Answer 6

1 drop of corQC Antiserum

Question 7

Centrifuge all tubes for

Answer 7

30 seconds

Question 8

Record results on the______

Answer 8

corQC reagent sheet

Question 9

Principle of ABO forward typing

Answer 9

Testing with both Anti-A and Anti-B is necessary to determine if red blood cells possess or lack A and/or B blood group antigens

Question 10

Absence of agglutination is a negative test result, which indicates______

Answer 10

the corresponding antigen is not demonstrable

Question 11

Agglutination of red blood cells with a given reagent is a positive test result, which indicates______

Answer 11

the presence of the corresponding antigen on the red blood cells

Question 12

To recognize reagent deterioration, the reagent must be_____

Answer 12

The reagent must be tested daily with appropriate controls

Question 13

Reagents contain _____as preservative

Answer 13

Sodium azide 0.1%

Question 14

Reagent should not be use beyond expiration date. T or F.

Answer 14

True

Question 15

Storage of the reagent

Answer 15

Store at 2 to 8C

May be at room temp (20 to 30C) while in use

Question 16

Specimen use in ABO forward typing tube method

Answer 16

EDTA anticoagulated blood

Question 17

In ABO forward typing, sample should be stored at_____

Answer 17

2 to 8 C

Question 18

No special preparation of the patient is required prior to specimen collection in ABO forward typing. T or F

Answer 18

True

Question 19

How many drops of buffered NSS in making 3-5% red cell suspension

Answer 19

19 drops

Question 20

How many drop/s of anti-A to tube “A”

Answer 20

1 drop

Question 21

How many drop/s of anti-B to tube “B”

Answer 21

1 drop

Question 22

How many drop/s of red cell suspension to the tubes “A” and “B”

Answer 22

1 drop

Question 23

_____________ of red blood cells in the presence of reagent is a positive test result and indicates the presence of the corresponding antigen.

Answer 23

Agglutination

Question 24

Negative control should have a ______

Answer 24

Negative result

Question 25

State the common sources of technical error in ABO typing:

Answer 25

1. Inadequate identification of blood specimens, test tubes or slides 2. Cell suspension either too heavy or too light 3. Clerical errors 4. A mix-up in samples 5. Missed observation of hemolysis 6. Failure to add reagents 7. Failure to follow manufacturer’s instructions 8. Uncalibrated centrifuge 9. Contaminated reagents/ expired reagents 10. Warming during centrifugation due to uncalibrated centrifuge

Question 26

One solid agglutinate (clear background)

Answer 26

+4

Question 27

Several large agglutinates (clear background)

Answer 27

+3

Question 28

Medium sized agglutinates

Answer 28

+2

Question 29

Small agglutinates (turbid background) Tiny agglutinates (turbid background)

Answer 29

+1 to W+

Question 30

No agglutination or hemolysis

Answer 30

0