BB LAB (reverse typing & Rh typing) MIDTERMS Flashcards
Give the principle of ABO reverse typing
Reverse (serum) grouping is designed to check for the presence of the expected ABO blood group antibodies by testing the serum of the patient with a pool of known A (A1) cells and known B cells.
To ensure accurate testing, it is advisable to ____________
To use freshly prepared 2-5% RBC suspension of a known blood type A and blood type B individual for A cell and B cell reagents respectively
Marked hemolysis indicates____________ and reagent must be _______
Instability or contamination; reagent must be discarded
Plasma can be used but ______ is preferred
Serum
T or F. There is patient special preparation for ABO reverse typing
False
How many drops of A1 cells to tube aC
1 drop
How many drop/s of B cells to tube bC
1 drop
How many drop/s of serum or plasma to tube aC and bC?
2 drops
Centrifuge for
30 seconds
What to examine after centrifugation?
Agglutination
____________ of the red blood cells in the presence of reagent is a positive test result for reverse typing
Agglutination
Agglutination in reverse typing indicates the presence of ________
Indicates the presence of the corresponding antibody
No agglutination if the red blood cells is a _________ and indicates_______
A negative test result; indicates the corresponding antibody is not demonstrable
Testing with anti-D reagent is necessary to________________
To determine if red blood cells possess or lack the D blood group antigen
Agglutination of red blood cells with anti-D reagent is a _______
Positive result
Absence of agglutination is a _____
Negative result
To recognized reagent deterioration, the reagent must be ________
Must be tested daily with appropriate controls
T or F. Reagent must be use beyond expiry date
False.
Reagent must not be use beyond expiry date
Reagent must be stored at
2 to 8 C
Reagent must be stored within this temperature while in use
Room temp. (20 - 30C)
Specimen for Rh blood typing
EDTA or Oxalated blood is preferred
How many drop/s of buffered NSS in Rh typing?
19 drops
How many drop/s of washed red cells should you put in the tube in Rh typing
1 drop
How many drop/s of anti-D to tube “D”
1 drop
Agglutination in RH typing is a _______
Positive test result and indicates the presence of D antigen.
No agglutination in Rh typing is ________
A negative test result and indicates absence of D antigen.
If the patient is female with a child-bearing age or possible donor, in Rh typing, before reporting it as negative this has to be confirmed by_____
Doing another procedure before reporting as negative
Rh antigens are located on the____
Surface of the red blood cells
T or F. Rh antigens are found exclusively on the RBCs and are not found on tissue cells or in soluble form in the body fluids.
True
The biochemical nature of the Rh antigens is_______
Lipoprotein
These antigens are an integral part of the red blood cell membrane
Rh antigens
What is the primary antigen in Rh antigen?
D antigen
When D antigen is present on RBCs, the individual is designated as ______
Rh positive
In what year the D antigen was discovered
1941
Who discovered the D antigen
Dr. Karl Landsteiner and Dr. Alexander S. Weiner
Both doctors used the term “Rh” in honor of the _______ they used in their experiment
Rhesus monkey
Most of the people are _____
Rh positive
These people are Rh negative
Caucasians
