BB LAB (reverse typing & Rh typing) MIDTERMS

Question 1

Give the principle of ABO reverse typing

Answer 1

Reverse (serum) grouping is designed to check for the presence of the expected ABO blood group antibodies by testing the serum of the patient with a pool of known A (A1) cells and known B cells.

Question 2

To ensure accurate testing, it is advisable to ____________

Answer 2

To use freshly prepared 2-5% RBC suspension of a known blood type A and blood type B individual for A cell and B cell reagents respectively

Question 3

Marked hemolysis indicates____________ and reagent must be _______

Answer 3

Instability or contamination; reagent must be discarded

Question 4

Plasma can be used but ______ is preferred

Answer 4

Serum

Question 5

T or F. There is patient special preparation for ABO reverse typing

Answer 5

False

Question 6

How many drops of A1 cells to tube aC

Answer 6

1 drop

Question 7

How many drop/s of B cells to tube bC

Answer 7

1 drop

Question 8

How many drop/s of serum or plasma to tube aC and bC?

Answer 8

2 drops

Question 9

Centrifuge for

Answer 9

30 seconds

Question 10

What to examine after centrifugation?

Answer 10

Agglutination

Question 11

____________ of the red blood cells in the presence of reagent is a positive test result for reverse typing

Answer 11

Agglutination

Question 12

Agglutination in reverse typing indicates the presence of ________

Answer 12

Indicates the presence of the corresponding antibody

Question 13

No agglutination if the red blood cells is a _________ and indicates_______

Answer 13

A negative test result; indicates the corresponding antibody is not demonstrable

Question 14

Testing with anti-D reagent is necessary to________________

Answer 14

To determine if red blood cells possess or lack the D blood group antigen

Question 15

Agglutination of red blood cells with anti-D reagent is a _______

Answer 15

Positive result

Question 16

Absence of agglutination is a _____

Answer 16

Negative result

Question 17

To recognized reagent deterioration, the reagent must be ________

Answer 17

Must be tested daily with appropriate controls

Question 18

T or F. Reagent must be use beyond expiry date

Answer 18

False.
Reagent must not be use beyond expiry date

Question 19

Reagent must be stored at

Answer 19

2 to 8 C

Question 20

Reagent must be stored within this temperature while in use

Answer 20

Room temp. (20 - 30C)

Question 21

Specimen for Rh blood typing

Answer 21

EDTA or Oxalated blood is preferred

Question 22

How many drop/s of buffered NSS in Rh typing?

Answer 22

19 drops

Question 23

How many drop/s of washed red cells should you put in the tube in Rh typing

Answer 23

1 drop

Question 24

How many drop/s of anti-D to tube “D”

Answer 24

1 drop

Question 25

Agglutination in RH typing is a _______

Answer 25

Positive test result and indicates the presence of D antigen.

Question 26

No agglutination in Rh typing is ________

Answer 26

A negative test result and indicates absence of D antigen.

Question 27

If the patient is female with a child-bearing age or possible donor, in Rh typing, before reporting it as negative this has to be confirmed by_____

Answer 27

Doing another procedure before reporting as negative

Question 28

Rh antigens are located on the____

Answer 28

Surface of the red blood cells

Question 29

T or F. Rh antigens are found exclusively on the RBCs and are not found on tissue cells or in soluble form in the body fluids.

Answer 29

True

Question 30

The biochemical nature of the Rh antigens is_______

Answer 30

Lipoprotein

Question 31

These antigens are an integral part of the red blood cell membrane

Answer 31

Rh antigens

Question 32

What is the primary antigen in Rh antigen?

Answer 32

D antigen

Question 33

When D antigen is present on RBCs, the individual is designated as ______

Answer 33

Rh positive

Question 34

In what year the D antigen was discovered

Answer 34

1941

Question 35

Who discovered the D antigen

Answer 35

Dr. Karl Landsteiner and Dr. Alexander S. Weiner

Question 36

Both doctors used the term “Rh” in honor of the _______ they used in their experiment

Answer 36

Rhesus monkey

Question 37

Most of the people are _____

Answer 37

Rh positive

Question 38

These people are Rh negative

Answer 38

Caucasians